"RIV/00216224:14330/08:00027029!RIV10-MSM-14330___" . "cDNA Microarray: Gene Expression Profiling of Small Cell Populations" . "Hrab\u010D\u00E1kov\u00E1, Viera" . "cDNA Microarray: Gene Expression Profiling of Small Cell Populations" . . . "cDNA microarray; RNA isolation; RNA amplifiacation; CD34+ cells"@en . . . . "Klabusay, Martin" . . . . "cDNA microarray technology is widely used in various biological and medical disciplines to determine gene expression profiles. Unfortunately, this technology requires a large quantity of input RNA. Since there is an increasing need to analyze more precisely defined cell sub-populations with low cell counts, working protocols using a minimal number of input cells are needed. Optimal RNA isolation and its accurate amplification are crucial to the success of these protocols. The goal was to find the best method of isolating and amplifying RNA from a small number of cells. We used the HL60 cell line in the search for a suitable protocol that could be applied to clinical samples of CD34+ hematopoietic cells. These cells make up only a very small population in bone marrow (1- 4% of all cells)." . "358970" . "cDNA Microarray: Gene Expression Profiling of Small Cell Populations"@en . "cDNA Microarray: Gene Expression Profiling of Small Cell Populations"@en . "RIV/00216224:14330/08:00027029" . "cDNA microarray technology is widely used in various biological and medical disciplines to determine gene expression profiles. Unfortunately, this technology requires a large quantity of input RNA. Since there is an increasing need to analyze more precisely defined cell sub-populations with low cell counts, working protocols using a minimal number of input cells are needed. Optimal RNA isolation and its accurate amplification are crucial to the success of these protocols. The goal was to find the best method of isolating and amplifying RNA from a small number of cells. We used the HL60 cell line in the search for a suitable protocol that could be applied to clinical samples of CD34+ hematopoietic cells. These cells make up only a very small population in bone marrow (1- 4% of all cells)."@en . . "7"^^ . . "14330" . "Tesa\u0159ov\u00E1, Lenka" . . "[40A2CA0B2606]" . . . . . . . . . "7"^^ . . "Ru\u010Dka, Zden\u011Bk" . . "Pot\u011B\u0161ilov\u00E1, Michaela" . "P(2B06052), P(LC535), Z(MSM0021622430)" . . "Peterkov\u00E1, Martina" . "Krontor\u00E1d Koutn\u00E1, Irena" . . . .