"14330" . . . "P(GP204/03/D031), P(LC535), Z(MSM0021622419)" . "1"^^ . . . . "Analytical Cytometry IV: Book of Abstracts" . "Kozubek, Michal" . "Fluorescent protein technology has started by using aequorea victoria fluorescent protein derivatives. These have become the essential tools for the cell biology research. Enhanced green fluorescent protein (EGFP) has been mutated to cyan (ECFP) and yellow (EYFP) fluorescent variants, to allow the simultaneous observation of several protein fusions in live cells especially for co-localization studies and to use the biophysical properties of these fluorophores to detect protein-protein interactions and conformational changes by fluorescence resonance energy transfer (FRET) technique. The discovery and development of red fluorescent protein variants have led to fluorophores that can be distinctly imaged together with other fluorescent proteins to achieve two- or three-color imaging."@en . "fluorescence; microscopy; living cells; image analysis"@en . . . . "RIV/00216224:14330/07:00020659!RIV10-GA0-14330___" . . . "6"^^ . . "Amrichov\u00E1, Jana" . . "Fluorescent microscopy of living cells"@en . "6"^^ . "978-80-239-9591-6" . . . "Ka\u0161\u00EDk, Marek" . "Fluorescent microscopy of living cells" . . "Va\u0159echa, Miroslav" . "[D190582B7B97]" . . "Brno" . . "Brno" . "Fluorescent microscopy of living cells" . "Fluorescent microscopy of living cells"@en . . "2007-06-23+02:00"^^ . . . "Fluorescent protein technology has started by using aequorea victoria fluorescent protein derivatives. These have become the essential tools for the cell biology research. Enhanced green fluorescent protein (EGFP) has been mutated to cyan (ECFP) and yellow (EYFP) fluorescent variants, to allow the simultaneous observation of several protein fusions in live cells especially for co-localization studies and to use the biophysical properties of these fluorophores to detect protein-protein interactions and conformational changes by fluorescence resonance energy transfer (FRET) technique. The discovery and development of red fluorescent protein variants have led to fluorophores that can be distinctly imaged together with other fluorescent proteins to achieve two- or three-color imaging." . . "RIV/00216224:14330/07:00020659" . . . . "Zimmermann, Michal" . "Ulman, Vladim\u00EDr" . . "\u010Cesk\u00E1 spole\u010Dnost pro analytickou cytologii" . "422266" .