"Bioinformatic predictions and image analysis of localization and interactions of endonuclease G, AIF, and AMID in human cells" . "6"^^ . . . . . "Bioinformatic predictions and image analysis of localization and interactions of endonuclease G, AIF, and AMID in human cells"@en . . . . "[411C706571A8]" . "Kozubek, Michal" . . . "RIV/00216224:14330/07:00020613" . "Ulman, Vladim\u00EDr" . "Zimmermann, Michal" . "411849" . . . . "P(GP204/05/P090), P(LC535), Z(MSM0021622419)" . . "Amrichov\u00E1, Jana" . "During apoptosis several mitochondrial proteins are released. They participate in caspase-independent nuclear DNA degradation, namely apoptosis-inducing factor (AIF, also PDCD8 or AIFM1) and endonuclease G. Another interesting protein, which is not located inside mitochondria, was expected to act similarly as AIF due to high sequence homology with AIF. This protein is AIF-homologous mitochondrion-associated inducer of death (AMID, also PRG3 or AIFM2). We studied the cellular localization and colocalization of proteins AIF, endonuclease G and AMID experimentally using designed mammalian expression vectors, which carry genes encoding the proteins of interest fused to the fluorescent proteins and using bioinformatic predictions, that analyze the amino acid sequence of the proteins with various algorithms. We also designed and applied the novel method of single-cell image analysis of the translocation of the fluorescent proteins into the nucleus." . . . . . "During apoptosis several mitochondrial proteins are released. They participate in caspase-independent nuclear DNA degradation, namely apoptosis-inducing factor (AIF, also PDCD8 or AIFM1) and endonuclease G. Another interesting protein, which is not located inside mitochondria, was expected to act similarly as AIF due to high sequence homology with AIF. This protein is AIF-homologous mitochondrion-associated inducer of death (AMID, also PRG3 or AIFM2). We studied the cellular localization and colocalization of proteins AIF, endonuclease G and AMID experimentally using designed mammalian expression vectors, which carry genes encoding the proteins of interest fused to the fluorescent proteins and using bioinformatic predictions, that analyze the amino acid sequence of the proteins with various algorithms. We also designed and applied the novel method of single-cell image analysis of the translocation of the fluorescent proteins into the nucleus."@en . . . "Bioinformatic predictions and image analysis of localization and interactions of endonuclease G, AIF, and AMID in human cells"@en . . "14330" . "RIV/00216224:14330/07:00020613!RIV11-GA0-14330___" . "Va\u0159echa, Miroslav" . . "6"^^ . . "Matula, Pavel" . . "Bioinformatic predictions and image analysis of localization and interactions of endonuclease G, AIF, and AMID in human cells" . "apoptosis; cell death; autophagy; cancer"@en . .