"14330" . "\u0160majs, David" . "RIV/00216224:14330/07:00020336" . "P(GA310/07/0321), P(NR8967), Z(MSM0021622415)" . . "C\u00EDl: Srovnat komparativn\u00ED genomovou sekvenaci (CGS) a pyrosekvenaci a ur\u010Dit spr\u00E1vnou sekvenci genomu Treponema pallidum ssp. pallidum Samoa D. Materi\u00E1l a metody: Metoda CGS umo\u017E\u0148uje sekvenaci bl\u00EDzce p\u0159\u00EDbuzn\u00FDch mikrobi\u00E1ln\u00EDch genom\u016F s vyu\u017Eit\u00EDm znalosti referen\u010Dn\u00EDho genomu. Pyrosekvencov\u00E1n\u00ED je metoda umo\u017E\u0148uj\u00EDc\u00ED sekvenaci cel\u00E9ho mikrobi\u00E1ln\u00EDho genomu de novo i z mal\u00E9ho mno\u017Estv\u00ED DNA bez nutnosti konstrukce genomov\u00E9 knihovny. Pyrosekvencov\u00E1n\u00ED vyu\u017E\u00EDv\u00E1 luminiscen\u010Dn\u00ED detekce bisfosf\u00E1tu uvoln\u011Bn\u00E9ho p\u0159i prodlu\u017Eov\u00E1n\u00ED replikovan\u00E9ho \u0159et\u011Bzce inkorporac\u00ED nukleotid\u016F polymer\u00E1zou. Mezi sekvencemi z CGS a pyrosekvencov\u00E1n\u00ED bylo odhaleno 512 odli\u0161nost\u00ED, a to p\u0159ev\u00E1\u017En\u011B jednonukleotidov\u00FDch. Ty byly rozd\u011Bleny do 345 oblast\u00ED, kter\u00E9 byly navr\u017Eeny pro amplifikaci PCR a enzymatickou sekvenaci. Z v\u00FDsledk\u016F srovn\u00E1n\u00ED vypl\u00FDv\u00E1, \u017Ee CGS chybuje asi 1,2x \u010Dast\u011Bji ne\u017E pyrosekvencov\u00E1n\u00ED. Na chyb\u00E1ch zp\u016Fsoben\u00FDch pyrosekvencov\u00E1n\u00EDm se nejv\u00EDce pod\u00EDl\u00ED delece a inzerce (indely) a to celkem z 92,3 %."@cs . . "C\u00EDl: Srovnat komparativn\u00ED genomovou sekvenaci (CGS) a pyrosekvenaci a ur\u010Dit spr\u00E1vnou sekvenci genomu Treponema pallidum ssp. pallidum Samoa D. Materi\u00E1l a metody: Metoda CGS umo\u017E\u0148uje sekvenaci bl\u00EDzce p\u0159\u00EDbuzn\u00FDch mikrobi\u00E1ln\u00EDch genom\u016F s vyu\u017Eit\u00EDm znalosti referen\u010Dn\u00EDho genomu. Pyrosekvencov\u00E1n\u00ED je metoda umo\u017E\u0148uj\u00EDc\u00ED sekvenaci cel\u00E9ho mikrobi\u00E1ln\u00EDho genomu de novo i z mal\u00E9ho mno\u017Estv\u00ED DNA bez nutnosti konstrukce genomov\u00E9 knihovny. Pyrosekvencov\u00E1n\u00ED vyu\u017E\u00EDv\u00E1 luminiscen\u010Dn\u00ED detekce bisfosf\u00E1tu uvoln\u011Bn\u00E9ho p\u0159i prodlu\u017Eov\u00E1n\u00ED replikovan\u00E9ho \u0159et\u011Bzce inkorporac\u00ED nukleotid\u016F polymer\u00E1zou. Mezi sekvencemi z CGS a pyrosekvencov\u00E1n\u00ED bylo odhaleno 512 odli\u0161nost\u00ED, a to p\u0159ev\u00E1\u017En\u011B jednonukleotidov\u00FDch. Ty byly rozd\u011Bleny do 345 oblast\u00ED, kter\u00E9 byly navr\u017Eeny pro amplifikaci PCR a enzymatickou sekvenaci. Z v\u00FDsledk\u016F srovn\u00E1n\u00ED vypl\u00FDv\u00E1, \u017Ee CGS chybuje asi 1,2x \u010Dast\u011Bji ne\u017E pyrosekvencov\u00E1n\u00ED. Na chyb\u00E1ch zp\u016Fsoben\u00FDch pyrosekvencov\u00E1n\u00EDm se nejv\u00EDce pod\u00EDl\u00ED delece a inzerce (indely) a to celkem z 92,3 %." . . . "[4DA10307B967]" . . . . "451879" . . . "2"^^ . "CGS; pyrosequencing; Treponema pallidum subspecies pertenue Samoa D"@en . . "Srovn\u00E1n\u00ED komparativn\u00ED genomov\u00E9 sekvenace a pyrosekvenace u Treponema pallidum subspecies pertenue Samoa D" . "Zoban\u00EDkov\u00E1, Marie" . . "Srovn\u00E1n\u00ED komparativn\u00ED genomov\u00E9 sekvenace a pyrosekvenace u Treponema pallidum subspecies pertenue Samoa D"@cs . "2"^^ . . "CGS and pyrosequencing of Treponema pallidum subspecies pertenue Samoa D"@en . "CGS and pyrosequencing of Treponema pallidum subspecies pertenue Samoa D"@en . "Srovn\u00E1n\u00ED komparativn\u00ED genomov\u00E9 sekvenace a pyrosekvenace u Treponema pallidum subspecies pertenue Samoa D" . . . . . . . . "RIV/00216224:14330/07:00020336!RIV10-MZ0-14330___" . "Srovn\u00E1n\u00ED komparativn\u00ED genomov\u00E9 sekvenace a pyrosekvenace u Treponema pallidum subspecies pertenue Samoa D"@cs . "CGS and pyrosequencing methods used for whole geneme sequencing of Treponema pallidum subspecies pertenue Samoa D are compared."@en .