"10"^^ . . "RIV/00216224:14330/07:00020185" . "Kozubek, Stanislav" . "0862-8408" . "Physiological Research" . . . . . . "Distinct patterns of histone methylation and acetylation in human interphase nuclei" . "B\u00E1rtov\u00E1, Eva" . "6" . . "Svoboda, David" . . "P(GA204/06/0978), P(GP202/03/D033), P(LC535), Z(AV0Z50040507), Z(AV0Z50040702), Z(MSM0021622419)" . . "14330" . . . "Skaln\u00EDkov\u00E1, Magdalena" . . . "56" . "CZ - \u010Cesk\u00E1 republika" . "Harni\u010Darov\u00E1, Andrea" . . "Distinct patterns of histone methylation and acetylation in human interphase nuclei" . "RIV/00216224:14330/07:00020185!RIV10-GA0-14330___" . "8"^^ . "8"^^ . "417591" . "Distinct patterns of histone methylation and acetylation in human interphase nuclei"@en . . . . . "Histone methylation; acetylation; X chromosome; centromere; image analysis"@en . . . "Matula, Petr" . "To study 3D nuclear distributions of epigenetic histone modifications such as H3(K9) acetylation, H3(K4) dimethylation, H3(K9) dimethylation, and H3(K27) trimethylation, and of histone methyl transferase Suv39H1, we used advanced image analysis methods, combined with Nipkow disk confocal microscopy. Total fluorescence intensity and distributions of fluorescently labelled proteins were analysed in formaldehyde-fixed interphase nuclei. Our data showed reduced fluorescent signals of H3(K9) acetylation and H3(K4) dimethylation (dime) at the nuclear periphery, while di-meH3(K9) was also abundant in chromatin regions closely associated with the nuclear envelope. Little overlapping (intermingling) was observed for di-meH3(K4) and H3(K27) trimethylation (tri-me), and for di-meH3(K9) and Suv39H1." . . . . "Ulman, Vladim\u00EDr" . "[C8953F712645]" . . . . "Distinct patterns of histone methylation and acetylation in human interphase nuclei"@en . . . "To study 3D nuclear distributions of epigenetic histone modifications such as H3(K9) acetylation, H3(K4) dimethylation, H3(K9) dimethylation, and H3(K27) trimethylation, and of histone methyl transferase Suv39H1, we used advanced image analysis methods, combined with Nipkow disk confocal microscopy. Total fluorescence intensity and distributions of fluorescently labelled proteins were analysed in formaldehyde-fixed interphase nuclei. Our data showed reduced fluorescent signals of H3(K9) acetylation and H3(K4) dimethylation (dime) at the nuclear periphery, while di-meH3(K9) was also abundant in chromatin regions closely associated with the nuclear envelope. Little overlapping (intermingling) was observed for di-meH3(K4) and H3(K27) trimethylation (tri-me), and for di-meH3(K9) and Suv39H1."@en . . "Kozubek, Michal" . .