. . . . . "Je\u017Eek, Jan" . . "P(IAA1065203)" . "International Society for Optical Engineering" . "Sledov\u00E1n\u00ED trajektorie mikroobjekt\u016F uvnit\u0159 \u017Eiv\u00FDch bun\u011Bk"@cs . "3"^^ . . "Zem\u00E1nek, Pavel" . "\u010Ci\u017Em\u00E1r, Tom\u00E1\u0161" . "Amrichov\u00E1, Jana" . "618025-1" . . "Sledov\u00E1n\u00ED ciz\u00EDho objektu uvnit\u0159 \u017Eiv\u00FDch bun\u011Bk, bez naru\u0161en\u00ED struktury a \u017Eivotaschopnosti bun\u011Bk. Pomoc\u00ED lipozom\u016F se do bun\u011Bk p\u0159irozen\u011Bn p\u0159enesly fluorescen\u010Dn\u00ED mikrokuli\u010Dky, u kter\u00FDch jsme pak sledovali samovoln\u00FD tepeln\u00FD pohyb t\u011Bchto objekt\u016F s pomoc\u00ED video-mikroskopie."@cs . "Tracing of foreign objects inside living cells is very exciting way how to study interior of living objects in nondestructive way. We imported fluorescent submicron particles into the living cells using liposomes as carriers to study the local mechanical heterogeneity of the cell cytoplasm. Thermal motion of these probes within the cell is tracked using fluorescent video-microscopy. The time-records of the probe positions reveal their trajectories and accessible space to the probes inside the cytoplasm of living cells. Further analyses of the thermal motion of the probes can reveal the mechanism of sub-cellular transport and properties of the cytoplasm in vivo."@en . "0277-786X" . . "Optical tracking of microobjects within living cells"@en . . "RIV/00216224:14330/06:00015327" . . . . "Optical tracking of microobjects within living cells"@en . "14330" . "6"^^ . "Sledov\u00E1n\u00ED trajektorie mikroobjekt\u016F uvnit\u0159 \u017Eiv\u00FDch bun\u011Bk"@cs . "Kozubek, Michal" . "Bellingham, USA" . . "Praha" . "6"^^ . . . . . "Va\u0159echa, Miroslav" . "490743" . "[6084C6CC3802]" . "living cells; liposomes; optical microscopy; visualisation; optical tracking"@en . "Tracing of foreign objects inside living cells is very exciting way how to study interior of living objects in nondestructive way. We imported fluorescent submicron particles into the living cells using liposomes as carriers to study the local mechanical heterogeneity of the cell cytoplasm. Thermal motion of these probes within the cell is tracked using fluorescent video-microscopy. The time-records of the probe positions reveal their trajectories and accessible space to the probes inside the cytoplasm of living cells. Further analyses of the thermal motion of the probes can reveal the mechanism of sub-cellular transport and properties of the cytoplasm in vivo." . . "Optical tracking of microobjects within living cells" . "Proceedings of SPIE" . . "2005-01-01+01:00"^^ . "Optical tracking of microobjects within living cells" . "RIV/00216224:14330/06:00015327!RIV07-AV0-14330___" .