. . "Pachern\u00EDk, J." . . "Skaln\u00EDkov\u00E1, Magdalena" . "RIV/00216224:14330/05:00012958" . . . . "9"^^ . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . . "Kova\u0159\u00EDk, A." . . "Nuclear levels and patterns of histone H3 modification and HP1 proteins after inhibition of histone deacetylases" . "0021-9533" . . . "Nuclear levels and patterns of histone H3 modification and HP1 proteins after inhibition of histone deacetylases"@en . "6"^^ . "118" . "Journal of Cell Science" . . . "Kova\u0159\u00EDkov\u00E1, M." . "RIV/00216224:14330/05:00012958!RIV11-GA0-14330___" . . . "The effects of the histone deacetylase inhibitors (HDACi) trichostatin A (TSA) and sodium butyrate (NaBt) were studied in A549, HT29 and FHC human cell lines. Global histone hyperacetylation, leading to decondensation of interphase chromatin, was characterized by an increase in H3(K9) and H3(K4) dimethylation and H3(K9) acetylation. The levels of all isoforms of heterochromatin protein, HP1, were reduced after HDAC inhibition. The observed changes in the protein levels were accompanied by changes in their interphase patterns. In control cells, H3(K9) acetylation and H3(K4) dimethylation were substantially reduced to a thin layer at the nuclear periphery, whereas TSA and NaBt caused the peripheral regions to become intensely acetylated at H3(K9) and dimethylated at H3(K4). The dispersed pattern of H3(K9) dimethylation was stable even at the nuclear periphery of HDACi-treated cells." . . "P(1A8241), P(1QS500040508), P(GA202/04/0907), P(GP202/03/D033), P(IAA5004306), P(LC535), Z(AV0Z50040507)" . . . "533446" . "histone dimethylation; histone acetylation; HP1 proteins; HDAC inhibitors; nuclear periphery"@en . "The effects of the histone deacetylase inhibitors (HDACi) trichostatin A (TSA) and sodium butyrate (NaBt) were studied in A549, HT29 and FHC human cell lines. Global histone hyperacetylation, leading to decondensation of interphase chromatin, was characterized by an increase in H3(K9) and H3(K4) dimethylation and H3(K9) acetylation. The levels of all isoforms of heterochromatin protein, HP1, were reduced after HDAC inhibition. The observed changes in the protein levels were accompanied by changes in their interphase patterns. In control cells, H3(K9) acetylation and H3(K4) dimethylation were substantially reduced to a thin layer at the nuclear periphery, whereas TSA and NaBt caused the peripheral regions to become intensely acetylated at H3(K9) and dimethylated at H3(K4). The dispersed pattern of H3(K9) dimethylation was stable even at the nuclear periphery of HDACi-treated cells."@en . . "12"^^ . "14330" . "[E5B6D6569CC1]" . . "Harni\u010Darov\u00E1, Andrea" . "B\u00E1rtov\u00E1, Eva" . "Kozubek, Michal" . "Hofmanov\u00E1, Ji\u0159ina" . . . . . . "Nuclear levels and patterns of histone H3 modification and HP1 proteins after inhibition of histone deacetylases" . "Nuclear levels and patterns of histone H3 modification and HP1 proteins after inhibition of histone deacetylases"@en . "21" . . "Kozubek, Stanislav" . . .