"Colocalization of PML bodies and PML/RARalpha microspeckles with up- and down-regulated loci and changes of chromatin structure in APL leukemia cells"@en . "Kozubek, Michal" . "16"^^ . . . "80-210-3560-9" . "14330" . "Faretta, Mario" . "8"^^ . "Pelicci, Giuseppe" . . "Falk, Martin" . "RIV/00216224:14330/04:00009698" . "RIV/00216224:14330/04:00009698!RIV11-AV0-14330___" . "[382F3E315613]" . "The reciprocal translocation between PML and RARalpha genes associated with acute promyelocytic leukemia (APL) results in disintegration of PML bodies and expression of the fusion protein PML/RARalpha in transformed APL cells. Transcription intensity of particular genes were compared between Zn-treated (APL) and untreated (healthy) U937 PIR9 cells, stably transfected by the expression vector carrying the PML/RARalpha fusion gene under the control of Zn-inducible promoter; downregulation of many genes clustered in the locus 19p13.3 and upregulation of some of those in Xq28-Xqter were revealed by microchip analysis and RT-PCR after zinc treatment. We have examined the colocalization of these up- (Xq28-qter)-regulated (19p13.3)chromosomal loci with PML bodies in normal and PML/RARalpha microspeckles in transformed APL cells respectively, as well as the changes in chromatin structure of these loci induced in APL cells by PML/RARalpha-altered transcription."@en . "P(GA202/02/0804), P(IAA1065203), P(IAA5004306), P(IBS5004010), P(ME 565), P(NC6987), Z(AV0Z5004920)" . "Kozubek, Stanislav" . . . . . "The reciprocal translocation between PML and RARalpha genes associated with acute promyelocytic leukemia (APL) results in disintegration of PML bodies and expression of the fusion protein PML/RARalpha in transformed APL cells. Transcription intensity of particular genes were compared between Zn-treated (APL) and untreated (healthy) U937 PIR9 cells, stably transfected by the expression vector carrying the PML/RARalpha fusion gene under the control of Zn-inducible promoter; downregulation of many genes clustered in the locus 19p13.3 and upregulation of some of those in Xq28-Xqter were revealed by microchip analysis and RT-PCR after zinc treatment. We have examined the colocalization of these up- (Xq28-qter)-regulated (19p13.3)chromosomal loci with PML bodies in normal and PML/RARalpha microspeckles in transformed APL cells respectively, as well as the changes in chromatin structure of these loci induced in APL cells by PML/RARalpha-altered transcription." . . "Colocalization of PML bodies and PML/RARalpha microspeckles with up- and down-regulated loci and changes of chromatin structure in APL leukemia cells" . "558052" . . "Cytometry"@en . "Luk\u00E1\u0161ov\u00E1, Emilie" . . . "Colocalization of PML bodies and PML/RARalpha microspeckles with up- and down-regulated loci and changes of chromatin structure in APL leukemia cells" . "Brno" . . "Colocalization of PML bodies and PML/RARalpha microspeckles with up- and down-regulated loci and changes of chromatin structure in APL leukemia cells"@en . "Brno" . . "2004-10-12+02:00"^^ . . . . "Rochi, Mariano" . . . "Biophysics of the Genome" . "Dellino, Ivan" . . . "Masaryk University" . "2"^^ . . .