. "Hlohovec, Czech Republic" . . "P(IAB5004102), Z(MSM 143300002)" . . "Gajdu\u0161kov\u00E1, Pavla" . "2003-01-01+01:00"^^ . "Masaryk University" . . "5"^^ . "Brno" . . "598062" . "5"^^ . . . "Skaln\u00EDkov\u00E1, Magdalena" . . "3"^^ . . "14330" . . "Epigenetic silencing in eukaryotes is associated with the formation of a transcriptional repressive higher order chromatin structure which is also characterized by methylation of DNA at cytosine nucleotides. DNA methylation in eukaryotes involves of a methyl group to the carbon -5 position of the cytosine ring (5-mC). This reaction is catalysed by DNA methyltransferase in the context of the sequence 5'-GC-3', which is also referred to as a CpG dinucleotide. Differential methylation of CpG islands has been inversely correlated with gene activity: transcriptionally active genes were found to be hypomethylated, whereas transcriptionally silent genes were hypermethylated (Bird, A. 1992, Razin A. et al., 1991, Singal R., 1999). In our experiments in situ hybridisation technique and high-resolution cytometry were used to determine selected metaphase chromosomes HSA 1, 10, 18, 19 and 20." . . . . "B\u00E1rtov\u00E1, Eva" . . . . . . "Epigenetic silencing in eukaryotes is associated with the formation of a transcriptional repressive higher order chromatin structure which is also characterized by methylation of DNA at cytosine nucleotides. DNA methylation in eukaryotes involves of a methyl group to the carbon -5 position of the cytosine ring (5-mC). This reaction is catalysed by DNA methyltransferase in the context of the sequence 5'-GC-3', which is also referred to as a CpG dinucleotide. Differential methylation of CpG islands has been inversely correlated with gene activity: transcriptionally active genes were found to be hypomethylated, whereas transcriptionally silent genes were hypermethylated (Bird, A. 1992, Razin A. et al., 1991, Singal R., 1999). In our experiments in situ hybridisation technique and high-resolution cytometry were used to determine selected metaphase chromosomes HSA 1, 10, 18, 19 and 20."@en . "Analysis of DNA methylation status of cell nuclei using high-resolution" . . "Analysis of DNA methylation status of cell nuclei using high-resolution"@en . "80-210-3226-X" . "Kozubek, Michal" . "Biophysics of the Genome" . "Analysis of DNA methylation status of cell nuclei using high-resolution" . "Kozubek, Stanislav" . . "RIV/00216224:14330/03:00009009" . "[F4E48DE84259]" . "RIV/00216224:14330/03:00009009!RIV11-AV0-14330___" . "chromatin structure"@en . "Analysis of DNA methylation status of cell nuclei using high-resolution"@en .