. . . "14330" . . . "Fojtov\u00E1, Miloslava" . . "CH - \u0160v\u00FDcarsk\u00E1 konfederace" . . "5"^^ . "Kozubek, Stanislav" . . . "Gajdu\u0161kov\u00E1, Pavla" . "[2FC057776B21]" . . . . "Chromosomal territory segmentation in apoptotic cells" . "5" . . . "Sou\u010Dek, Karel" . "4"^^ . "Chromosomal territory segmentation in apoptotic cells"@en . . "60" . . . "979-990" . "Chromosomal territory segmentation in apoptotic cells"@cs . "RIV/00216224:14330/03:00008143" . "Chromosomal territory segmentation in apoptotic cells"@cs . . "601221" . "12"^^ . "Cellular and Molecular Life Sciences" . "RIV/00216224:14330/03:00008143!RIV08-AV0-14330___" . . . . "P(GA301/01/0186), P(IAA5004306), P(IAB5004102), P(IBS5004010), Z(AV0Z5004920), Z(MSM 143300002)" . "Chromosomal territory segmentation in apoptotic cells" . . "The nuclear architecture of selected chromosomes in apoptotic nuclei of human leukemic cells K-562 and HL-60 was investigated in the presented paper. Etoposide and prolonged confluence were used for the induction of apoptosis. DAPI- as well as TUNEL- labelling of apoptotic nuclear bodies was combined with visualisation of chromosomal territories by the FISH technique. Simultaneous vital staining by Annexin V, PI and Hoechst 33342 was applied in order to distinguish apoptotic, necrotic and intact cell fraction of tested population. Our FISH analyses revealed that the 3D- structure of apoptotic nuclei as well as the 3D- structure of apoptotic bodies is preserved in formaldehyde fixed cells. High molecular weight DNA fragmentation was determined in apoptotic K-562 cells in contrast to oligonucleosomal cleavage observed in apoptotic HL-60 cells. In K-562 population, chromosomal territories were located separately either in one apoptotic body or to undergo disassembly into chromosomal segments dispersed in" . . . "The nuclear architecture of selected chromosomes in apoptotic nuclei of human leukemic cells K-562 and HL-60 was investigated in the presented paper. Etoposide and prolonged confluence were used for the induction of apoptosis. DAPI- as well as TUNEL- labelling of apoptotic nuclear bodies was combined with visualisation of chromosomal territories by the FISH technique. Simultaneous vital staining by Annexin V, PI and Hoechst 33342 was applied in order to distinguish apoptotic, necrotic and intact cell fraction of tested population. Our FISH analyses revealed that the 3D- structure of apoptotic nuclei as well as the 3D- structure of apoptotic bodies is preserved in formaldehyde fixed cells. High molecular weight DNA fragmentation was determined in apoptotic K-562 cells in contrast to oligonucleosomal cleavage observed in apoptotic HL-60 cells. In K-562 population, chromosomal territories were located separately either in one apoptotic body or to undergo disassembly into chromosomal segments dispersed in"@cs . "1420-682X" . "Chromosomal territory; Nuclear architecture; Apoptosis; DNA fragmentation"@en . . "Chromosomal territory segmentation in apoptotic cells"@en . "The nuclear architecture of selected chromosomes in apoptotic nuclei of human leukemic cells K-562 and HL-60 was investigated in the presented paper. Etoposide and prolonged confluence were used for the induction of apoptosis. DAPI- as well as TUNEL- labelling of apoptotic nuclear bodies was combined with visualisation of chromosomal territories by the FISH technique. Simultaneous vital staining by Annexin V, PI and Hoechst 33342 was applied in order to distinguish apoptotic, necrotic and intact cell fraction of tested population. Our FISH analyses revealed that the 3D- structure of apoptotic nuclei as well as the 3D- structure of apoptotic bodies is preserved in formaldehyde fixed cells. High molecular weight DNA fragmentation was determined in apoptotic K-562 cells in contrast to oligonucleosomal cleavage observed in apoptotic HL-60 cells. In K-562 population, chromosomal territories were located separately either in one apoptotic body or to undergo disassembly into chromosomal segments dispersed in"@en . "B\u00E1rtov\u00E1, Eva" .