. . . . . . . . . . "14310" . "31" . "5"^^ . "glioblastoma multiforme; epidermal growth factor receptor signaling; PDGFR; TAM receptors; receptor tyrosine kinase; mitogen-activated protein kinase"@en . "Zitterbart, Karel" . "8"^^ . . "5"^^ . . . . "10.3892/or.2013.2864" . . "13882" . "RIV/00216224:14310/14:00074892" . . . "EGFR signaling in the HGG-02 glioblastoma cell line with an unusual loss of EGFR gene copy"@en . "Veselsk\u00E1, Renata" . . "Epidermal growth factor receptor (EGFR) gene amplification and the overexpression of EGFR are described as common features of glioblastoma multiforme (GBM). Nevertheless, we previously reported the loss of EGFR gene copy in a GBM specimen from a patient with an unusually favorable course of the disease, and the HGG-02 cell line with this aberration was successfully derived from this tumor. Here, we present a detailed analysis of changes in gene expression and cell signaling in the HGG-02 cell line; the GM7 reference cell line with a standard EGFR gene copy number derived from a very aggressive GBM was used as a control. We confirmed the downregulation of EGFR expression and signaling in HGG-02 cells using different methods (RTK analysis, gene profiling and RT-PCR). Other changes that may have contributed to the non-aggressive phenotype of the primary tumor were identified, including the downregulated phosphorylation of the Axl and Trk receptors, as well as increased activity of JNK and p38 kinases."@en . "\u0160koda, Jan" . "EGFR signaling in the HGG-02 glioblastoma cell line with an unusual loss of EGFR gene copy" . "[8DB74939EF87]" . "\u0160t\u011Brba, Jaroslav" . "EGFR signaling in the HGG-02 glioblastoma cell line with an unusual loss of EGFR gene copy"@en . "RIV/00216224:14310/14:00074892!RIV15-MSM-14310___" . "000330788100065" . "P(ED2.1.00/03.0101), P(EE2.3.20.0183)" . . "Epidermal growth factor receptor (EGFR) gene amplification and the overexpression of EGFR are described as common features of glioblastoma multiforme (GBM). Nevertheless, we previously reported the loss of EGFR gene copy in a GBM specimen from a patient with an unusually favorable course of the disease, and the HGG-02 cell line with this aberration was successfully derived from this tumor. Here, we present a detailed analysis of changes in gene expression and cell signaling in the HGG-02 cell line; the GM7 reference cell line with a standard EGFR gene copy number derived from a very aggressive GBM was used as a control. We confirmed the downregulation of EGFR expression and signaling in HGG-02 cells using different methods (RTK analysis, gene profiling and RT-PCR). Other changes that may have contributed to the non-aggressive phenotype of the primary tumor were identified, including the downregulated phosphorylation of the Axl and Trk receptors, as well as increased activity of JNK and p38 kinases." . "1021-335X" . "Neradil, Jakub" . "Oncology Reports" . "EGFR signaling in the HGG-02 glioblastoma cell line with an unusual loss of EGFR gene copy" . . . . "1" . . "GR - \u0158eck\u00E1 republika" .