. . . . . "RIV/00216224:14310/14:00074241" . . "Wimmerov\u00E1, Michaela" . . . . "49318" . "Targeted-random mutagenesis of PA-IIL lectin and high-throughput screening of mutant library" . "3"^^ . . . "3"^^ . . . . "lectins; PA-IIL; Pseudomonas aeruginosa; targeted-random mutagenesis; high-throughput screening; mutant library"@en . . "PA-IIL lectin from Pseudomonas aeruginosa shows a unique binding mode among bacterial lectins because it binds saccharides through two calcium cations. Its features make this protein a good candidate for tuning its affinity and specificity. Two amino acids responsible for sugar binding were chosen for targeted-random mutagenesis. If this approach is used, it is necessary to utilize high-throughput screening of mutant libraries for identification of mutants with interestingly modified specificity and/or affinity."@en . . "P(GA13-25401S), S" . "14310" . "RIV/00216224:14310/14:00074241!RIV15-MSM-14310___" . . "PA-IIL lectin from Pseudomonas aeruginosa shows a unique binding mode among bacterial lectins because it binds saccharides through two calcium cations. Its features make this protein a good candidate for tuning its affinity and specificity. Two amino acids responsible for sugar binding were chosen for targeted-random mutagenesis. If this approach is used, it is necessary to utilize high-throughput screening of mutant libraries for identification of mutants with interestingly modified specificity and/or affinity." . "Targeted-random mutagenesis of PA-IIL lectin and high-throughput screening of mutant library"@en . . "Targeted-random mutagenesis of PA-IIL lectin and high-throughput screening of mutant library"@en . "Mr\u00E1zkov\u00E1, Jana" . . "[6789249D2788]" . "Targeted-random mutagenesis of PA-IIL lectin and high-throughput screening of mutant library" . . . "Pokorn\u00E1, Martina" .