"Lloyd-Lewis, Bethan" . . . "Science Signaling" . . "[6781110E8862]" . . "7" . "RIV/00216224:14310/14:00073568!RIV15-MSM-14310___" . "Huwe1-mediated ubiquitylation of dishevelled defines a negative feedback loop in the wnt signaling pathway."@en . "Ganji, Sri Ranjani" . "11"^^ . "de Groot, Reinoud E. A." . "Ganji, Sri Ranjani" . "000333557500002" . . "10.1126/scisignal.2004985" . "5"^^ . . . "Wnt signaling plays a central role in development, adult tissue homeostasis, and cancer. Several steps in the canonical Wnt/beta-catenin signaling cascade are regulated by ubiquitylation, a protein modification that influences the stability, subcellular localization, or interactions of target proteins. To identify regulators of the Wnt/beta-catenin pathway, we performed an RNA interference screen in Caenorhabditis elegans and identified the HECT domain-containing ubiquitin ligase EEL-1 as an inhibitor of Wnt signaling. In human embryonic kidney 293T cells, knockdown of the EEL-1 homolog Huwe1 enhanced the activity of a Wnt reporter in cells stimulated with Wnt3a or in cells that overexpressed casein kinase 1 (CK1) or a constitutively active mutant of the Wnt co-receptor low-density lipoprotein receptor-related protein 6 (LRP6)." . "I, P(GA204/09/0498), P(GD204/09/H058), S, Z(MSM0021622430)" . . . . . "\u0160edov\u00E1, Kate\u0159ina" . "Huwe1-mediated ubiquitylation of dishevelled defines a negative feedback loop in the wnt signaling pathway." . "Seipel, Katja" . "Wnt signaling plays a central role in development, adult tissue homeostasis, and cancer. Several steps in the canonical Wnt/beta-catenin signaling cascade are regulated by ubiquitylation, a protein modification that influences the stability, subcellular localization, or interactions of target proteins. To identify regulators of the Wnt/beta-catenin pathway, we performed an RNA interference screen in Caenorhabditis elegans and identified the HECT domain-containing ubiquitin ligase EEL-1 as an inhibitor of Wnt signaling. In human embryonic kidney 293T cells, knockdown of the EEL-1 homolog Huwe1 enhanced the activity of a Wnt reporter in cells stimulated with Wnt3a or in cells that overexpressed casein kinase 1 (CK1) or a constitutively active mutant of the Wnt co-receptor low-density lipoprotein receptor-related protein 6 (LRP6)."@en . "Dale, Trevor C" . . "Korswagen, Hendrik C." . "317" . "10"^^ . "1937-9145" . "Huwe1-mediated ubiquitylation of dishevelled defines a negative feedback loop in the wnt signaling pathway." . "14310" . "Dhople, Vishnu M." . "Bryja, V\u00EDt\u011Bzslav" . . . . . "RIV/00216224:14310/14:00073568" . . "Huwe1-mediated ubiquitylation of dishevelled defines a negative feedback loop in the wnt signaling pathway."@en . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Huwe1-mediated ubiquitylation of dishevelled defines a negative feedback loop in the wnt signaling pathway."@en . "Bernat\u00EDk, Ond\u0159ej" . "Zdr\u00E1hal, Zbyn\u011Bk" . . "20019" . . .