"Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity"@en . "Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity" . "Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis and Steinernema are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. These EPNs are symbiotically associated with bacteria of the genera Photorhabdus or Xenorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest its tissues to provide nutrients for themselves and for expanding nematodes. We used EPNs infection to study immunity of Drosophila melanogaster and here we show for the first time that also honeybee larvae are suitable hosts for nematobacterial complex. We used the tripartite model (honeybee, nematodes, bacteria) to develop standard procedure for testing honeybees' immune response. We optimised the infection for EPN Heterorhabditis bacteriophora and Steinernema feltiae; both species cause typical coloration of cadavers (red or yellow, respectively), multiply in honeybee larvae and shows similar mortality with LD50 approx."@en . "RIV/00216224:14310/14:00073256!RIV15-MZE-14310___" . "Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity"@en . "Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis and Steinernema are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. These EPNs are symbiotically associated with bacteria of the genera Photorhabdus or Xenorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest its tissues to provide nutrients for themselves and for expanding nematodes. We used EPNs infection to study immunity of Drosophila melanogaster and here we show for the first time that also honeybee larvae are suitable hosts for nematobacterial complex. We used the tripartite model (honeybee, nematodes, bacteria) to develop standard procedure for testing honeybees' immune response. We optimised the infection for EPN Heterorhabditis bacteriophora and Steinernema feltiae; both species cause typical coloration of cadavers (red or yellow, respectively), multiply in honeybee larvae and shows similar mortality with LD50 approx." . "Infection of honeybee larvae by entomopathogenic nematodes - natural model to study honeybee immunity" . . "entomopathogenic nematodes; honeaybee; Photorhabdus luminescens; Heterorhabditis bacteriophora"@en . "21270" . . . . . "Hyr\u0161l, Pavel" . . "Berka, Jakub" . . "P(QJ1210047)" . "RIV/00216224:14310/14:00073256" . "4"^^ . . . "14310" . . "3"^^ . "Dobe\u0161, Pavel" . . . . "Vojtek, Libor" . . "[8A2089D4B824]" . . . . .