. "[171C7478E72E]" . . "7108" . . "Phagocytosis is one of the most important innate immunity mechanisms which prevents organism against pathogens overcoming the natural barriers. There are several methods widely used for evaluation of phagocytosis efficiency. One of them is to measure the level of reactive oxygen species (ROS) produced by phagocytes - respiratory burst of phagocytes. ROS production can be elicited by addition of activators such as lipopolysaccharide (LPS), zymosan, starch particles, phorbol-12-miristate-13-acetate (PMA) or N-formyl-methionyl-leucyl-phenylalanin (fMLP). Produced ROS cause oxidation of luminophore which subsequently emits the energy in form of light measured by luminometers. Luminol is the most widely used luminophore. On the other hand evaluation of respiratory burst using this method on birds or insect samples is not possible due to a low production of ROS and low sensitivity of luminol." . . . . "RIV/00216224:14310/14:00073254!RIV15-MSM-14310___" . . "Chemiluminescent determination of reactive oxygen species using Pholasin luminophore in birds and insect phagocytes"@en . . "Chemiluminescent determination of reactive oxygen species using Pholasin luminophore in birds and insect phagocytes" . "Chemiluminescent determination of reactive oxygen species using Pholasin luminophore in birds and insect phagocytes" . "Vinkler, Michal" . . "Prokopov\u00E1, Lucie" . "I, P(QJ1210047)" . . "RIV/00216224:14310/14:00073254" . "14310" . . . . . "6"^^ . . "Chemiluminiscence; phagocytes; Pholasin; reactive oxygen species"@en . "Vinklerova, Jitka" . . . . "Phagocytosis is one of the most important innate immunity mechanisms which prevents organism against pathogens overcoming the natural barriers. There are several methods widely used for evaluation of phagocytosis efficiency. One of them is to measure the level of reactive oxygen species (ROS) produced by phagocytes - respiratory burst of phagocytes. ROS production can be elicited by addition of activators such as lipopolysaccharide (LPS), zymosan, starch particles, phorbol-12-miristate-13-acetate (PMA) or N-formyl-methionyl-leucyl-phenylalanin (fMLP). Produced ROS cause oxidation of luminophore which subsequently emits the energy in form of light measured by luminometers. Luminol is the most widely used luminophore. On the other hand evaluation of respiratory burst using this method on birds or insect samples is not possible due to a low production of ROS and low sensitivity of luminol."@en . "4"^^ . . "Vojtek, Libor" . . "Dobe\u0161, Pavel" . "Chemiluminescent determination of reactive oxygen species using Pholasin luminophore in birds and insect phagocytes"@en . "Hyr\u0161l, Pavel" .