. "Method of Thermostabilization of a Protein And/or Stabilization Towards Organic Solvents"@en . . . . . "Alexandria" . . . "Thermostabilization; Protein Stabilization; Organic Solvents"@en . . . . "Chovancov\u00E1, Eva" . . "MU" . "I, P(ED0001/01/01)" . . "RIV/00216224:14310/13:00072736" . "\u0160t\u011Bp\u00E1nkov\u00E1, Veronika" . . . . "RIV/00216224:14310/13:00072736!RIV14-MSM-14310___" . "Chaloupkov\u00E1, Radka" . "Brezovsk\u00FD, Jan" . "Method of Thermostabilization of a Protein And/or Stabilization Towards Organic Solvents" . . "[B87F20DF72ED]" . . . "Koudel\u00E1kov\u00E1, T\u00E1\u0148a" . . . "The present invention relates to a method of thermostabilization of a protein and/or stabilization of a protein towards organic solvents wherein the protein contains access routes, wherein at least one amino acid in the bottleneck of the access route of the protein is mutated. Preferably, the method comprises the steps of identification of amino acids forming the bottleneck of the access routes, wherein said amino acids control the exchange of the solvent between the buried protein core and the surrounding environment and/or in the packing of the amino acids inside the access route; modification of the amino acids determined in the previous step so that the packing of the amino acids inside the tunnel is improved and the access route prevents the access of the undesired solvent molecules to the protein core, while it allows the passage of the compounds necessary at the protein core to enable the protein to perform its biological function."@en . . "6"^^ . "8"^^ . "Damborsk\u00FD, Ji\u0159\u00ED" . "Method of Thermostabilization of a Protein And/or Stabilization Towards Organic Solvents" . "14310" . "2013-11-12+01:00"^^ . . . "87569" . . . . "United States Patent and Trademark Office (USPTO)" . . "The present invention relates to a method of thermostabilization of a protein and/or stabilization of a protein towards organic solvents wherein the protein contains access routes, wherein at least one amino acid in the bottleneck of the access route of the protein is mutated. Preferably, the method comprises the steps of identification of amino acids forming the bottleneck of the access routes, wherein said amino acids control the exchange of the solvent between the buried protein core and the surrounding environment and/or in the packing of the amino acids inside the access route; modification of the amino acids determined in the previous step so that the packing of the amino acids inside the tunnel is improved and the access route prevents the access of the undesired solvent molecules to the protein core, while it allows the passage of the compounds necessary at the protein core to enable the protein to perform its biological function." . "Gora, Artur Wiktor" . "US 8,580,932" . "Prokop, Zbyn\u011Bk" . . "Method of Thermostabilization of a Protein And/or Stabilization Towards Organic Solvents"@en .