. "Ferrous iron oxidation by sulfur-oxidizing Acidithiobacillus ferrooxidans and analysis of the process at the levels of transcription and protein synthesis" . . . . . "0003-6072" . . "74903" . . . . "Bouchal, Pavel" . . . . . "7"^^ . "Ferrous iron oxidation by sulfur-oxidizing Acidithiobacillus ferrooxidans and analysis of the process at the levels of transcription and protein synthesis" . "103" . "7"^^ . . "Acidithiobacillus ferrooxidans; elemental sulfur oxidation; ferrous iron oxidation; pyrite oxidation; proteomics; reverse-transcription quantitative PCR"@en . "NL - Nizozemsko" . . "In contrast to iron-oxidizing Acidithiobacillus ferrooxidans, A. ferrooxidans from a stationary phase elemental sulfur-oxidizing culture exhibited a lag phase in pyrite oxidation, which is similar to its behavior during ferrous iron oxidation. The ability of elemental sulfur-oxidizing A. ferrooxidans to immediately oxidize ferrous iron or pyrite without a lag phase was only observed in bacteria obtained from growing cultures with elemental sulfur. However, these cultures that shifted to ferrous iron oxidation showed a low rate of ferrous iron oxidation while no growth was observed. Two-dimensional gel electrophoresis was used for a quantitative proteomic analysis of the adaptation process when bacteria were switched from elemental sulfur to ferrous iron. A comparison of total cell lysates revealed 39 proteins whose increase or decrease in abundance was related to this phenotypic switching. However, only a few proteins were closely related to iron and sulfur metabolism." . "P(ED2.1.00/03.0101), P(GA525/08/0697)" . "Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology" . . . . . "Janiczek, Old\u0159ich" . . "RIV/00216224:14310/13:00066107" . . . "Mandl, Martin" . . "Ferrous iron oxidation by sulfur-oxidizing Acidithiobacillus ferrooxidans and analysis of the process at the levels of transcription and protein synthesis"@en . "10.1007/s10482-012-9872-2" . . . "Ku\u010Dera, Ji\u0159\u00ED" . "4" . "14310" . "Lochman, Jan" . "In contrast to iron-oxidizing Acidithiobacillus ferrooxidans, A. ferrooxidans from a stationary phase elemental sulfur-oxidizing culture exhibited a lag phase in pyrite oxidation, which is similar to its behavior during ferrous iron oxidation. The ability of elemental sulfur-oxidizing A. ferrooxidans to immediately oxidize ferrous iron or pyrite without a lag phase was only observed in bacteria obtained from growing cultures with elemental sulfur. However, these cultures that shifted to ferrous iron oxidation showed a low rate of ferrous iron oxidation while no growth was observed. Two-dimensional gel electrophoresis was used for a quantitative proteomic analysis of the adaptation process when bacteria were switched from elemental sulfur to ferrous iron. A comparison of total cell lysates revealed 39 proteins whose increase or decrease in abundance was related to this phenotypic switching. However, only a few proteins were closely related to iron and sulfur metabolism."@en . . "Pot\u011B\u0161il, David" . "Ferrous iron oxidation by sulfur-oxidizing Acidithiobacillus ferrooxidans and analysis of the process at the levels of transcription and protein synthesis"@en . "RIV/00216224:14310/13:00066107!RIV14-GA0-14310___" . "000316145900021" . "Zdr\u00E1hal, Zbyn\u011Bk" . "15"^^ . "[B59908162DFB]" .