. . "MU, I\u010CO00216224" . . "RIV/00216224:14310/12:00064442!RIV13-MSM-14310___" . "3"^^ . . "14310" . . "Thermostable haloalkane dehalogenase DhaA88 prepared by site-directed mutagenesis. Mutations targeting as few as four residues lining the access tunnel extended enzyme\u2019s half-life in 40% dimethyl sulfoxide from minutes to weeks (4,000-fold) and increased its melting temperature by 19 C. Protein crystallography and molecular dynamics revealed that the tunnel residue packing is a key determinant of protein stability and the active-site accessibility for co-solvent molecules (red dots). The broad applicability of this concept was verified by analyzing twenty six proteins with buried active sites from all six enzyme classes.The results was made based on the verification of properties." . . "Damborsk\u00FD, Ji\u0159\u00ED" . . "Koudel\u00E1kov\u00E1, T\u00E1\u0148a" . "RIV/00216224:14310/12:00064442" . "Engineered recombinant enzyme haloalkane dehalogenase DhaA88"@en . "4"^^ . . . "Engineered recombinant enzyme haloalkane dehalogenase DhaA88" . "[EE5A1B5D3C23]" . . "Prokop, Zbyn\u011Bk" . . "Engineered recombinant enzyme haloalkane dehalogenase DhaA88" . . "Engineered recombinant enzyme haloalkane dehalogenase DhaA88"@en . . "Thermostable haloalkane dehalogenase DhaA88 prepared by site-directed mutagenesis. Mutations targeting as few as four residues lining the access tunnel extended enzyme\u2019s half-life in 40% dimethyl sulfoxide from minutes to weeks (4,000-fold) and increased its melting temperature by 19 C. Protein crystallography and molecular dynamics revealed that the tunnel residue packing is a key determinant of protein stability and the active-site accessibility for co-solvent molecules (red dots). The broad applicability of this concept was verified by analyzing twenty six proteins with buried active sites from all six enzyme classes.The results was made based on the verification of properties."@en . . . "P(ED0001/01/01)" . . . "134405" . . . "Chaloupkov\u00E1, Radka" . . "v\u00FDrobn\u00ED postup, funk\u010Dn\u00ED vzorek byl vyroben na z\u00E1klad\u011B ov\u011B\u0159en\u00ED vlastnost\u00ED, licen\u010Dn\u00ED smlouva nebyla zat\u00EDm uzav\u0159ena." . . "haloalkane dehalogenase; DhaA88"@en . "LL_07_2012" .