. "Forsbeg, A." . "Francisella tularensis; glycosylation; FTH_0069; DsbA; PilA; O-antigen; mass spectrometry"@en . "000306411300004" . "Characterization of Protein Glycosylation in Francisella tularensis subsp. holarctica"@en . . . . . "[84C8117FF1DB]" . . . "1535-9476" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Balonov\u00E1, Lucie" . . . . . . ": FTH_0069 is a previously uncharacterized strongly immunoreactive protein that has been proposed to be a novel virulence factor in Francisella tularensis. Here, the glycan structure modifying two C-terminal peptides of FTH_0069 was identified utilizing high resolution, high mass accuracy mass spectrometry, combined with in-source CID tandem MS experiments. The glycan observed at m/z 1156 was determined to be a hexasaccharide, consisting of two hexoses, three N-acetylhexosamines, and an unknown monosaccharide containing a phosphate group. The monosaccharide sequence of the glycan is tentatively proposed as X-P-HexNAc-HexNAc-Hex-Hex-HexNAc, where X denotes the unknown monosaccharide. The glycan is identical to that of DsbA glycoprotein, as well as to one of the multiple glycan structures modifying the type IV pilin PilA, suggesting a common biosynthetic pathway for the protein modification." . . . "\u010Cerven\u00FD, Luk\u00E1\u0161" . ": FTH_0069 is a previously uncharacterized strongly immunoreactive protein that has been proposed to be a novel virulence factor in Francisella tularensis. Here, the glycan structure modifying two C-terminal peptides of FTH_0069 was identified utilizing high resolution, high mass accuracy mass spectrometry, combined with in-source CID tandem MS experiments. The glycan observed at m/z 1156 was determined to be a hexasaccharide, consisting of two hexoses, three N-acetylhexosamines, and an unknown monosaccharide containing a phosphate group. The monosaccharide sequence of the glycan is tentatively proposed as X-P-HexNAc-HexNAc-Hex-Hex-HexNAc, where X denotes the unknown monosaccharide. The glycan is identical to that of DsbA glycoprotein, as well as to one of the multiple glycan structures modifying the type IV pilin PilA, suggesting a common biosynthetic pathway for the protein modification."@en . . . "Damborsk\u00FD, Ji\u0159\u00ED" . "Solomonsson, E. N." . "7" . . "Characterization of Protein Glycosylation in Francisella tularensis subsp. holarctica"@en . . "Molecular and Cellurar Proteomic" . . "RIV/00216224:14310/12:00062587" . "12"^^ . "Henrychov\u00E1, Lenka" . "\u0160ebestov\u00E1, Eva" . . "Novotn\u00FD, L. V." . "Stul\u00EDk, Ji\u0159\u00ED" . "Characterization of Protein Glycosylation in Francisella tularensis subsp. holarctica" . . . "Mann, B. F." . . "11" . "Characterization of Protein Glycosylation in Francisella tularensis subsp. holarctica" . . "126832" . "10.1074/mcp.M111.015016" . "RIV/00216224:14310/12:00062587!RIV13-MSM-14310___" . "14310" . "Forslund, Anna-Lena" . . "I, P(GA203/09/0857), P(ME08105), P(NS9747), Z(MO0FVZ0000604)" . "12"^^ . "Alley, W.R.Jr" . "2"^^ . .