"Rotkov\u00E1, Gabriela" . "S" . . . "Kyse\u013E, Peter" . "4"^^ . "[80F7674A183A]" . . "RIV/00216224:14310/12:00060443" . . . . "4"^^ . . "Expression of mycobacterial Rv3782 galactofuranosyltransferase in fussion with MBP" . "Expression of mycobacterial Rv3782 galactofuranosyltransferase in fussion with MBP"@en . . . . . . "135885" . "cell wall; glycosyltransferase; galactofuranosyltransferase; Mycobacterium tuberculosis; protein expression; protein purification; tuberculosis"@en . "Expression of mycobacterial Rv3782 galactofuranosyltransferase in fussion with MBP" . . . . "Mycobacterium tuberculosis is a gram positive, acid resistent bacterium causing chronic illness. Tuberculosis is characterized by a disease persistence and overall difficulties associated with its treatment. A combination of the sophisticated defence barrier and the slow metabolism of the bacterium make it a difficult target for therapeutics. The mycobacterial cell wall is an essential, covalently linked complex of highly hydrophobic mycolic acids, peptidoglycan and heteropolymeric arabinogalactan. Arabinogalactan is composed of about 30 alternating 5- and 6-linked b-D-galactofuranose (Galf) units. It anchores in peptidoglycan by a dedicated linker unit, a-L-rhamnopyranosyl-(1-3)-N-acetyl-a-D-glucosaminyl-phosphate [Rha-(1-3)-GlcNAc-P]. Two to three arabinan chains containing approximately 23 D-arabinofuranose (Araf) units each, composed mostly of 5-linked a-D-Araf with branching introduced by 3,5-a-D-Araf, are bound to the galactan."@en . "14310" . "Wimmerov\u00E1, Michaela" . . "Kostl\u00E1nov\u00E1, Nikola" . . "RIV/00216224:14310/12:00060443!RIV13-MSM-14310___" . "Mycobacterium tuberculosis is a gram positive, acid resistent bacterium causing chronic illness. Tuberculosis is characterized by a disease persistence and overall difficulties associated with its treatment. A combination of the sophisticated defence barrier and the slow metabolism of the bacterium make it a difficult target for therapeutics. The mycobacterial cell wall is an essential, covalently linked complex of highly hydrophobic mycolic acids, peptidoglycan and heteropolymeric arabinogalactan. Arabinogalactan is composed of about 30 alternating 5- and 6-linked b-D-galactofuranose (Galf) units. It anchores in peptidoglycan by a dedicated linker unit, a-L-rhamnopyranosyl-(1-3)-N-acetyl-a-D-glucosaminyl-phosphate [Rha-(1-3)-GlcNAc-P]. Two to three arabinan chains containing approximately 23 D-arabinofuranose (Araf) units each, composed mostly of 5-linked a-D-Araf with branching introduced by 3,5-a-D-Araf, are bound to the galactan." . "Expression of mycobacterial Rv3782 galactofuranosyltransferase in fussion with MBP"@en . . . . . .