"7"^^ . "[684571D252F4]" . "0003-6072" . "Kinetics of anaerobic elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans and protein identification by comparative 2-DE-MS/MS"@en . "Pot\u011B\u0161il, David" . . . "Zdr\u00E1hal, Zbyn\u011Bk" . "RIV/00216224:14310/12:00057288!RIV13-GA0-14310___" . . "Elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans was investigated. The apparent Michaelis constant for ferric iron was 18.6 mM. An absence of anaerobic ferric iron reduction ability was observed in bacteria maintained on elemental sulfur for an extended period of time. Upon transition from ferrous iron to elemental sulfur medium, the cells exhibited similar kinetic characteristics of ferric iron reduction under anaerobic conditions to those of cells that were originally maintained on ferrous iron. Nevertheless, a total loss of anaerobic ferric iron reduction ability after the sixth passage in elemental sulfur medium was demonstrated. The first proteomic screening of total cell lysates of anaerobically incubated bacteria resulted in the detection of 1599 protein spots in the master two-dimensional electrophoresis gel." . . "Kinetics of anaerobic elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans and protein identification by comparative 2-DE-MS/MS" . . "144657" . . . "\u010Cern\u00E1, Hana" . . "Ku\u010Dera, Ji\u0159\u00ED" . . "Elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans was investigated. The apparent Michaelis constant for ferric iron was 18.6 mM. An absence of anaerobic ferric iron reduction ability was observed in bacteria maintained on elemental sulfur for an extended period of time. Upon transition from ferrous iron to elemental sulfur medium, the cells exhibited similar kinetic characteristics of ferric iron reduction under anaerobic conditions to those of cells that were originally maintained on ferrous iron. Nevertheless, a total loss of anaerobic ferric iron reduction ability after the sixth passage in elemental sulfur medium was demonstrated. The first proteomic screening of total cell lysates of anaerobically incubated bacteria resulted in the detection of 1599 protein spots in the master two-dimensional electrophoresis gel."@en . . . "Mandl, Martin" . . "Kinetics of anaerobic elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans and protein identification by comparative 2-DE-MS/MS"@en . . . . . "Janiczek, Old\u0159ich" . . "3" . "000300314200010" . . "P(GA525/08/0697), Z(MSM0021622413), Z(MSM0021622415)" . . . . "NL - Nizozemsko" . "RIV/00216224:14310/12:00057288" . "ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY" . . . "101" . "7"^^ . . "Bouchal, Pavel" . . . "14310" . "Kinetics of anaerobic elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans and protein identification by comparative 2-DE-MS/MS" . "13"^^ . "Acidithiobacillus; Anaerobic sulfur oxidation; Ferric iron reduction; Proteomics; MS analysis"@en . . "10.1007/s10482-011-9670-2" .