. . . "14310" . . "Metodologie stanoven\u00ED variability alel MHC gen\u016F u ryb \u010Deledi Cyprinidae" . "3"^^ . "4"^^ . "Metodologie stanoven\u00ED variability alel MHC gen\u016F u ryb \u010Deledi Cyprinidae"@cs . . . "Metodologie stanoven\u00ED variability alel MHC gen\u016F u ryb \u010Deledi Cyprinidae"@cs . "The methodology of variability determination in MHC genes in fish of family Cyprinidae"@en . "MHC genes; variability; CE-SSCP; 454 pyrosequencing; fish; Cyprinidae"@en . . . . . . . "212059" . "Metodologie stanoven\u00ED variability alel MHC gen\u016F u ryb \u010Deledi Cyprinidae" . "Civ\u00E1\u0148ov\u00E1, Krist\u00EDna" . "[5F2E97AB02D2]" . . "K anal\u00FDze variability exprimovan\u00FDch gen\u016F DAB1 a DAB3 u kaprovit\u00FDch ryb jsme pou\u017Eili 2 metody. Prvn\u00ED metoda zahrnuje SSCP anal\u00FDzu, klonov\u00E1n\u00ED PCR amplikon\u016F, a automatick\u00E9 sekvenov\u00E1n\u00ED. Jako druhou jsme optimalizovali p\u0159\u00EDpravu vzork\u016F pro \u201Eklon\u00E1ln\u00ED\u201C sekvenov\u00E1n\u00ED druh\u00E9 generace (NGS \u2013 next generation sequencing) s vyu\u017Eit\u00EDm technologie 454 pyrosekvenov\u00E1n\u00ED. V p\u0159\u00EDsp\u011Bvku srovn\u00E1v\u00E1me klady a z\u00E1pory n\u00E1mi pou\u017E\u00EDvan\u00FDch metod testov\u00E1n\u00ED." . "RIV/00216224:14310/11:00050361" . . "To analyze the variability of expressed DAB1 and DAB3 genes in cyprinid fish we used two methods. The first one includes SSCP analysis, cloning of PCR amplicons and automatic sequencing. As the second one we optimized the preparation of the samples for clonal next generation sequencing (NGS) with usage of technology of 454 pyrosequencing. In the present work we compare pros and cones of the described methods used for testing."@en . "P(GA524/07/0188), P(GAP505/10/1138), Z(MSM0021622416)" . "Hejlov\u00E1, Krist\u00FDna" . . . "K anal\u00FDze variability exprimovan\u00FDch gen\u016F DAB1 a DAB3 u kaprovit\u00FDch ryb jsme pou\u017Eili 2 metody. Prvn\u00ED metoda zahrnuje SSCP anal\u00FDzu, klonov\u00E1n\u00ED PCR amplikon\u016F, a automatick\u00E9 sekvenov\u00E1n\u00ED. Jako druhou jsme optimalizovali p\u0159\u00EDpravu vzork\u016F pro \u201Eklon\u00E1ln\u00ED\u201C sekvenov\u00E1n\u00ED druh\u00E9 generace (NGS \u2013 next generation sequencing) s vyu\u017Eit\u00EDm technologie 454 pyrosekvenov\u00E1n\u00ED. V p\u0159\u00EDsp\u011Bvku srovn\u00E1v\u00E1me klady a z\u00E1pory n\u00E1mi pou\u017E\u00EDvan\u00FDch metod testov\u00E1n\u00ED."@cs . . "RIV/00216224:14310/11:00050361!RIV12-GA0-14310___" . . . . . . "The methodology of variability determination in MHC genes in fish of family Cyprinidae"@en . . "Martin, Jean-Francois" . "Vete\u0161n\u00EDkov\u00E1 \u0160imkov\u00E1, Andrea" . .