"190638" . . . . "Cloning, expression and structure determination of SH3b cell wall binding domain of bacteriophage 812 endolysin"@en . "Pant\u016F\u010Dek, Roman" . "In this study we focused on endolysin of staphylococcal bacteriophage 812, a member of unclassified SPO1-like viruses from family Myoviridae. The endolysin of phage 812 includes three domains: two catalytic domains and the SH3b-domain that is probably a cell wall targeting domain, which could be responsible for binding of endolysin to peptidoglycan. The gene sequence for the C-terminal SH3b domain was cloned in pET28 vector and expressed as a soluble protein in E. coli BL21 (DE3) to determine the 3D structure of the protein. A native variant of the SH3b protein was purified without the his-tag. Subsequently, three variants of this protein have been prepared: (i) non-labeled, (ii) single-labeled (15N) and (iii) double-labeled (13C, 15N) domain. The protein was purified to homogeneity using ammonium sulphate precipitation, anion exchange chromatography, cation exchange chromatography, and gel filtration."@en . . "Janda, Lubom\u00EDr" . "Mo\u0161a, Marek" . . . . . "Cloning, expression and structure determination of SH3b cell wall binding domain of bacteriophage 812 endolysin" . "9"^^ . "RIV/00216224:14310/11:00050057!RIV12-TA0-14310___" . . "P(GA310/09/0459), P(GAP206/11/0758), P(GP204/02/D099), P(TA01010405), Z(MSM0021622415)" . . . "8"^^ . . . . . "Bene\u0161\u00EDk, Martin" . "bacteriophage therapy; Staphylococcus aureus; bacteriophage endolysin; SH3b domain"@en . . "RIV/00216224:14310/11:00050057" . . "14310" . . . "\u017D\u00EDdek, Luk\u00E1\u0161" . "[AA634D126475]" . "Dopitov\u00E1, Radka" . "Nov\u00E1\u010Dek, Ji\u0159\u00ED" . . . . "Cloning, expression and structure determination of SH3b cell wall binding domain of bacteriophage 812 endolysin"@en . . . "In this study we focused on endolysin of staphylococcal bacteriophage 812, a member of unclassified SPO1-like viruses from family Myoviridae. The endolysin of phage 812 includes three domains: two catalytic domains and the SH3b-domain that is probably a cell wall targeting domain, which could be responsible for binding of endolysin to peptidoglycan. The gene sequence for the C-terminal SH3b domain was cloned in pET28 vector and expressed as a soluble protein in E. coli BL21 (DE3) to determine the 3D structure of the protein. A native variant of the SH3b protein was purified without the his-tag. Subsequently, three variants of this protein have been prepared: (i) non-labeled, (ii) single-labeled (15N) and (iii) double-labeled (13C, 15N) domain. The protein was purified to homogeneity using ammonium sulphate precipitation, anion exchange chromatography, cation exchange chromatography, and gel filtration." . "R\u016F\u017Ei\u010Dkov\u00E1, Vladislava" . "Cloning, expression and structure determination of SH3b cell wall binding domain of bacteriophage 812 endolysin" . . "Do\u0161ka\u0159, Ji\u0159\u00ED" . . . . .