. . "Knopfov\u00E1, Lucia" . . "Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity"@en . . . "4"^^ . "189299" . . "RIV/00216224:14310/11:00049467" . . "3"^^ . . . "Jan\u010Dekov\u00E1, Blanka" . . "Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity" . . "[B97B7D39BD9B]" . "Bene\u0161, Petr" . "The lysosomal aspartic protease cathepsin D (cath-D) is often over-expressed in breast cancer and acts as a mitogen on both cancer and stromal cells. The ability of cath-D to stimulate cancer cell migration remains controversial. To determine whether cath-D expression modulates breast cancer cell migration in vitro, we performed a real-time analysis of MDA-MB-231 breast cancer cell migration using novel methodology based on impedance measurement that enables continuous monitoring of the transition of the cells through a microporous membrane [xCELLigence equipped with CIM-plate 16 (Roche)]. We observed that altering cath-D level by either siRNA or cDNA tranfections significantly affected migration of MDA-MB-231 cells. Tumor cells have often impaired classical caspase-dependent apoptosis pathway and may be therefore more sensitive to agents that trigger alternative cell death pathways. Targeting lysosomes represents a method of choice as many human tumors have increased levels of lysosomal proteases." . "Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity"@en . "Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity" . . . . . "The lysosomal aspartic protease cathepsin D (cath-D) is often over-expressed in breast cancer and acts as a mitogen on both cancer and stromal cells. The ability of cath-D to stimulate cancer cell migration remains controversial. To determine whether cath-D expression modulates breast cancer cell migration in vitro, we performed a real-time analysis of MDA-MB-231 breast cancer cell migration using novel methodology based on impedance measurement that enables continuous monitoring of the transition of the cells through a microporous membrane [xCELLigence equipped with CIM-plate 16 (Roche)]. We observed that altering cath-D level by either siRNA or cDNA tranfections significantly affected migration of MDA-MB-231 cells. Tumor cells have often impaired classical caspase-dependent apoptosis pathway and may be therefore more sensitive to agents that trigger alternative cell death pathways. Targeting lysosomes represents a method of choice as many human tumors have increased levels of lysosomal proteases."@en . "P(GA301/09/1115), P(GD204/08/H054), P(IAA501630801), Z(MSM0021622415)" . . . . . . . . "cathepsin D; cell migration; breast cancer"@en . "RIV/00216224:14310/11:00049467!RIV12-GA0-14310___" . "\u0160marda, Jan" . "14310" .