. . "7"^^ . . "Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine" . . "oligonucleotides; adenine; cytosine; protonation; thermodynamics; chemometrics"@en . . "8"^^ . "Lubal, P\u0159emysl" . . "0366-6352" . "6" . . "T\u00E1borsk\u00FD, Petr" . "Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine" . "Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine"@en . . "Dospivov\u00E1, Dana" . "[8A8F2F54BDA2]" . "RIV/00216224:14310/09:00037430" . "342909" . . . "Acidobasic properties of purine and pyrimidine bases (adenine, cytosine) and relevant nucleosides (adenosine, cytidine) were studied by means of glass-electrode potentiometry and the respective dissociation constants were determined under given experimental conditions (I = 0.1 M (NaCl), t = (25.0 +/- 0.1) degrees of Celsius): adenine (pK_HL = 9.65 +/- 0.04, pK_H2L = 4.18 +/- 0.04), adenosine (pK_H2L = 3.59 +/- 0.05), cytosine (pK_H2L = 4.56 +/- 0.01), cytidine (pK_H2L = 4.16 +/- 0.02). In addition, thermodynamic parameters for bases were calculated. Acidobasic behavior of oligonucleotides ((CAC)3, A3C6, C3A3C3) was studied under the same experimental conditions by molecular absorption spectroscopy. pH-dependent spectral datasets were analyzed by means of advanced chemometric techniques (EFA, MCR-ALS) and the presence of hemiprotonated species concerning (C-C+) a non-canonical pair (i-motif) in titled oligonucleotides was proposed in order to explain experimental data obtained according to literature."@en . . . . . "Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine"@en . . "P(LC06035), S" . "SK - Slovensk\u00E1 republika" . "Acidobasic properties of purine and pyrimidine bases (adenine, cytosine) and relevant nucleosides (adenosine, cytidine) were studied by means of glass-electrode potentiometry and the respective dissociation constants were determined under given experimental conditions (I = 0.1 M (NaCl), t = (25.0 +/- 0.1) degrees of Celsius): adenine (pK_HL = 9.65 +/- 0.04, pK_H2L = 4.18 +/- 0.04), adenosine (pK_H2L = 3.59 +/- 0.05), cytosine (pK_H2L = 4.56 +/- 0.01), cytidine (pK_H2L = 4.16 +/- 0.02). In addition, thermodynamic parameters for bases were calculated. Acidobasic behavior of oligonucleotides ((CAC)3, A3C6, C3A3C3) was studied under the same experimental conditions by molecular absorption spectroscopy. pH-dependent spectral datasets were analyzed by means of advanced chemometric techniques (EFA, MCR-ALS) and the presence of hemiprotonated species concerning (C-C+) a non-canonical pair (i-motif) in titled oligonucleotides was proposed in order to explain experimental data obtained according to literature." . . . . . "63" . . . "Trnkov\u00E1, Libu\u0161e" . "Chem. Papers" . "RIV/00216224:14310/09:00037430!RIV10-MSM-14310___" . . "Pila\u0159ov\u00E1, Iveta" . "Vojtylov\u00E1, Ter\u00E9zia" . . . "14310" . "Farkov\u00E1, Marta" . "T\u0159\u00EDskov\u00E1, Olga" . . . "8"^^ .