. "P(GP525/08/P464), Z(MSM0021622412)" . "In vitro assessment of AhR-mediated activities of TCDD in mixture with humic substances" . . "In vitro assessment of AhR-mediated activities of TCDD in mixture with humic substances" . . . "In vitro assessment of AhR-mediated activities of TCDD in mixture with humic substances"@en . . "318915" . . . "76" . "11" . . "Chemosphere" . "RIV/00216224:14310/09:00029898!RIV10-MSM-14310___" . "Natural organic matter; AhR; Mixture; Interaction"@en . "0045-6535" . . "Bittner, Michal" . . . "In vitro assessment of AhR-mediated activities of TCDD in mixture with humic substances"@en . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "4"^^ . "Hilscherov\u00E1, Kl\u00E1ra" . "John, Giesy" . . "[454F010A953C]" . "000270124800009" . "3"^^ . . . "RIV/00216224:14310/09:00029898" . . . "Humic substances (HS) are ubiquitous natural products of decomposition of dead organic matter. HS is present in most freshwaters at concentrations ranging from 0.5 to 50 mg L-1. Organic carbon can represent 20% dry weight of sediments. Recently, the interaction of dissolved HS with the aryl hydrocarbon receptor (AhR) has been demonstrated. The AhR is a cytosolic receptor to which persistent organic pollutants (POPs) can bind and many of their toxic effects are mediated through interactions with this receptor. We describe in vitro effects (using H4IIE-luc cells) of binary mixtures of various HS with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), since in contaminated environments these compounds occur simultaneously. Six out of 12 HS samples activated AhR even at environmentally relevant concentrations (17 mg L-1), but did not reach the full AhR-activation even at excessive concentration. In simultaneous exposure of H4IIE-luc to HS (17 mg L-1) and TCDD (1."@en . "2"^^ . . . "14310" . "Humic substances (HS) are ubiquitous natural products of decomposition of dead organic matter. HS is present in most freshwaters at concentrations ranging from 0.5 to 50 mg L-1. Organic carbon can represent 20% dry weight of sediments. Recently, the interaction of dissolved HS with the aryl hydrocarbon receptor (AhR) has been demonstrated. The AhR is a cytosolic receptor to which persistent organic pollutants (POPs) can bind and many of their toxic effects are mediated through interactions with this receptor. We describe in vitro effects (using H4IIE-luc cells) of binary mixtures of various HS with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), since in contaminated environments these compounds occur simultaneously. Six out of 12 HS samples activated AhR even at environmentally relevant concentrations (17 mg L-1), but did not reach the full AhR-activation even at excessive concentration. In simultaneous exposure of H4IIE-luc to HS (17 mg L-1) and TCDD (1." . .