. "RIV/00216224:14310/08:00026609!RIV10-MSM-14310___" . . . "397762" . . "Blanchard, Bertrand" . . "Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I" . . . "Hollwille, Emilie" . "The opportunistic pathogen Pseudomonas aeruginosa contains several carbohydrate-binding proteins, among which is the P. aeruginosa lectin I (PA-IL), which displays affinity for a-galactosylated glycans. Glycan arrays were screened and demonstrated stronger binding of PA-IL toward aGal1-4bGal-terminating structures and weaker binding to aGal1-3bGal ones in order to determine which human glycoconjugates could play a role in the carbohydrate-mediated adhesion of the bacteria. This was confirmed in vivo by testing the binding of the lectin to Burkitt lymphoma cells that present large amounts of globotriaosylceramide antigen Gb3/CD77/Pk. Trisaccharide moieties of Gb3 and isoglobotriaosylceramide were tested by titration microcalorimetry, and both displayed similar affinity to PA-IL in solution." . "Z(MSM0021622413)" . "NL - Nizozemsko" . "Journal of Molecular Biology" . "Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I" . "0022-2836" . "383" . . "2"^^ . "Nurisso, Alessandra" . "The opportunistic pathogen Pseudomonas aeruginosa contains several carbohydrate-binding proteins, among which is the P. aeruginosa lectin I (PA-IL), which displays affinity for a-galactosylated glycans. Glycan arrays were screened and demonstrated stronger binding of PA-IL toward aGal1-4bGal-terminating structures and weaker binding to aGal1-3bGal ones in order to determine which human glycoconjugates could play a role in the carbohydrate-mediated adhesion of the bacteria. This was confirmed in vivo by testing the binding of the lectin to Burkitt lymphoma cells that present large amounts of globotriaosylceramide antigen Gb3/CD77/Pk. Trisaccharide moieties of Gb3 and isoglobotriaosylceramide were tested by titration microcalorimetry, and both displayed similar affinity to PA-IL in solution."@en . . "Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I"@en . "Wimmerov\u00E1, Michaela" . . "Tetaud, Cecile" . "pseudomonas aeruginosa; lectin; molecular modeling; structure; glycosphinglipid; oligosaccharides"@en . "17"^^ . . "Structural Basis of the Preferential Binding for Globo-Series Glycosphingolipids Displayed by Pseudomonas aeruginosa Lectin I"@en . "9"^^ . "4" . . . "[4DD8367C569D]" . "14310" . . . . . . . "RIV/00216224:14310/08:00026609" . "Imberty, Anne" . "Varrotte, Annabelle" . "Pokorn\u00E1, Martina" . "000260533800009" . "Wiells, Joele" .