. "Wimmerov\u00E1, Michaela" . "Gene for Burkholderia cenocepacia lectin D (BclD) was cloned in pRSET vector and expressed. However, expression was difficult, with very low yield and protein was produced mainly in insoluble form. BclD contains N-terminal domain of unknown function. As expression of lectins domains are generally high, we decided to separate N-terminal and lectin domain. Several crystals of lectin domain in different conditions were obtained using hanging drop vapour diffusion technique. These crystals are ready for data collection at ESRF (European Synchrotron Radiation Facility)."@en . "[E2EC73703E9A]" . "358650" . "Materials Structure in Chemistry, Biology, Physics and Technology" . . "3a" . "Malinovsk\u00E1, Lenka" . . . . "15" . . "14310" . "P(GA303/06/0570), P(ME08008), Z(MSM0021622413)" . . "1211-5894" . "3"^^ . . . "Burkholderia cenocepacia lectin D"@en . "Gene for Burkholderia cenocepacia lectin D (BclD) was cloned in pRSET vector and expressed. However, expression was difficult, with very low yield and protein was produced mainly in insoluble form. BclD contains N-terminal domain of unknown function. As expression of lectins domains are generally high, we decided to separate N-terminal and lectin domain. Several crystals of lectin domain in different conditions were obtained using hanging drop vapour diffusion technique. These crystals are ready for data collection at ESRF (European Synchrotron Radiation Facility)." . "Burkholderia cenocepacia lectin D"@en . . . . "3"^^ . "Burkholderia cenocepacia lectin D" . . "CZ - \u010Cesk\u00E1 republika" . . . "Pokorn\u00E1, Marie" . "Burkholderia cenocepacia; lectin; structure"@en . "RIV/00216224:14310/08:00024966" . "2"^^ . . . "RIV/00216224:14310/08:00024966!RIV10-MSM-14310___" . "Burkholderia cenocepacia lectin D" . . . .