"Expression profiles of c-jun, c-fos and integrin beta 3 genes in differentiation of RAW264.7 cells."@en . "P(GA301/06/0036), P(GD204/08/H054), Z(MSM0021622415)" . "RIV/00216224:14310/08:00024706" . . "367404" . . "Na\u0161\u00EDm c\u00EDlem bylo stanovit dynamiku exprese gen\u016F c-jun, c-fos a itgb3 b\u011Bhem diferenciace bun\u011Bk RAW do osteoklast\u016F a dendritick\u00FDch bun\u011Bk. Genovou expresi jsme studovali na \u00FArovni RNA metodou kvantitativn\u00ED RT-PCR a na \u00FArovni protein\u016F westernov\u00FDm p\u0159enosem. Potvrdili jsme, \u017Ee b\u011Bhem diferenciace osteoklast\u016F v\u00FDrazn\u011B stoup\u00E1 exprese c-fos, zat\u00EDmco exprese c-jun postupn\u011B kles\u00E1. V dendritick\u00FDch bu\u0148k\u00E1ch je tomu p\u0159esn\u011B naopak, exprese c-jun stoup\u00E1 a c-fos kles\u00E1. Exprese itgb3 roste v pr\u016Fb\u011Bhu diferenciace jak osteoklast\u016F, tak dendritick\u00FDch bun\u011Bk."@cs . . "Na\u0161\u00EDm c\u00EDlem bylo stanovit dynamiku exprese gen\u016F c-jun, c-fos a itgb3 b\u011Bhem diferenciace bun\u011Bk RAW do osteoklast\u016F a dendritick\u00FDch bun\u011Bk. Genovou expresi jsme studovali na \u00FArovni RNA metodou kvantitativn\u00ED RT-PCR a na \u00FArovni protein\u016F westernov\u00FDm p\u0159enosem. Potvrdili jsme, \u017Ee b\u011Bhem diferenciace osteoklast\u016F v\u00FDrazn\u011B stoup\u00E1 exprese c-fos, zat\u00EDmco exprese c-jun postupn\u011B kles\u00E1. V dendritick\u00FDch bu\u0148k\u00E1ch je tomu p\u0159esn\u011B naopak, exprese c-jun stoup\u00E1 a c-fos kles\u00E1. Exprese itgb3 roste v pr\u016Fb\u011Bhu diferenciace jak osteoklast\u016F, tak dendritick\u00FDch bun\u011Bk." . "\u0160marda, Jan" . "RIV/00216224:14310/08:00024706!RIV11-GA0-14310___" . "c-jun; c-fos; integrin beta 3; RAW264.7; differentiation"@en . . . . "3"^^ . "Expresn\u00ED profily gen\u016F c-jun, c-fos a integrinu beta 3 v pr\u016Fb\u011Bhu diferenciace bun\u011Bk RAW264.7"@cs . "14310" . "Vop\u011Bnkov\u00E1, Kate\u0159ina" . "3"^^ . . . . "Va\u0148hara, Petr" . . . . . . . "Expresn\u00ED profily gen\u016F c-jun, c-fos a integrinu beta 3 v pr\u016Fb\u011Bhu diferenciace bun\u011Bk RAW264.7"@cs . . . . "[71ECB772FA24]" . "Expression profiles of c-jun, c-fos and integrin beta 3 genes in differentiation of RAW264.7 cells."@en . "Expresn\u00ED profily gen\u016F c-jun, c-fos a integrinu beta 3 v pr\u016Fb\u011Bhu diferenciace bun\u011Bk RAW264.7" . . "Expresn\u00ED profily gen\u016F c-jun, c-fos a integrinu beta 3 v pr\u016Fb\u011Bhu diferenciace bun\u011Bk RAW264.7" . . "The RAW 246.7 murine leukaemic monocyte/macrophage cell line possesses the ability to differentiate into different cell types, such as osteoclasts in RANKL and M-CSF dependent way or dendritic cells (by bacterial lipopolysaccharide). In this process, transcription factors c-Jun and c-Fos are necessary. Integrin beta 3 is important for function of osteoclasts as well by mediating adhesion to the bone surface and the bone matrix resorption. The promotor of integrin beta 3 gene contains binding sites for c-Jun and c-Fos transcription factors as described in in HD11 myeloblasts. Therefore, it is possible that crosstalk between these proteins exists and is important in regulation in osteoclasts activity or differentiation. These molecules can be important in dendritic cell differentiation as well. We studied the expression of c-jun, c-fos a itgB3 during the LPS or M-CSF-RANKL driven differentiation of RAW cells by using quantitative RT-PCR and western-blotting."@en . . .