. . "Bulletin \u010Cesk\u00E9 spole\u010Dnosti experiment\u00E1ln\u00ED biologie rostlin a Fyziologick\u00E9 sekce Slovensk\u00E9 botanick\u00E9 spole\u010Dnosti" . "V na\u0161\u00ED laborato\u0159i se zab\u00FDv\u00E1me studiem role rostlinn\u00FDch hormon\u016F, zejm\u00E9na auxinu, ve v\u00FDvoji modelov\u00E9ho organizmu Arabidopsis thaliana . D\u016Fle\u017Eit\u00FDm zji\u0161t\u011Bn\u00EDm posledn\u00EDch let byla identifikace PIN protein\u016F, kter\u00E9 jsou nezbytn\u00E9 pro transport auxinu z bun\u011Bk a jejich\u017E pol\u00E1rn\u00ED lokalizace v bu\u0148k\u00E1ch ur\u010Duje sm\u011Br mezibun\u011B\u010Dn\u00E9ho auxinov\u00E9ho toku. Dal\u0161\u00EDmi c\u00EDly je identifikace dosud nezn\u00E1m\u00FDch komponent auxinov\u00E9ho transportu a molekul\u00E1rn\u00EDch mechanism\u016F, kter\u00E9 \u0159\u00EDd\u00ED pol\u00E1rn\u00ED lokalizaci PIN protein\u016F. V populaci 35S::PIN1 se zv\u00FD\u0161enou expres\u00ED PIN1 proteinu, byly vyhled\u00E1v\u00E1ny mutace, kter\u00E9 potla\u010Duj\u00ED p\u016Fvodn\u00ED agravitropn\u00ED fenotypov\u00FD projev t\u00E9to transgenn\u00ED linie a v populaci PIN1-GFP s fluorescen\u010Dn\u011B zna\u010Den\u00FDm PIN1 proteinem, byly vyhled\u00E1v\u00E1ny mutace kter\u00E9 negativn\u011B ovliv\u0148uj\u00ED sm\u011Brov\u00E1n\u00ED PIN1-GFP na plazmatickou membr\u00E1nu." . "Identifikace a pozi\u010Dn\u00ED klonov\u00E1n\u00ED komponent auxinov\u00E9ho transportu a bun\u011B\u010Dn\u00E9 polarity u Arabidopsis thaliana" . "RIV/00216224:14310/07:00024018!RIV10-MSM-14310___" . "V na\u0161\u00ED laborato\u0159i se zab\u00FDv\u00E1me studiem role rostlinn\u00FDch hormon\u016F, zejm\u00E9na auxinu, ve v\u00FDvoji modelov\u00E9ho organizmu Arabidopsis thaliana . D\u016Fle\u017Eit\u00FDm zji\u0161t\u011Bn\u00EDm posledn\u00EDch let byla identifikace PIN protein\u016F, kter\u00E9 jsou nezbytn\u00E9 pro transport auxinu z bun\u011Bk a jejich\u017E pol\u00E1rn\u00ED lokalizace v bu\u0148k\u00E1ch ur\u010Duje sm\u011Br mezibun\u011B\u010Dn\u00E9ho auxinov\u00E9ho toku. Dal\u0161\u00EDmi c\u00EDly je identifikace dosud nezn\u00E1m\u00FDch komponent auxinov\u00E9ho transportu a molekul\u00E1rn\u00EDch mechanism\u016F, kter\u00E9 \u0159\u00EDd\u00ED pol\u00E1rn\u00ED lokalizaci PIN protein\u016F. V populaci 35S::PIN1 se zv\u00FD\u0161enou expres\u00ED PIN1 proteinu, byly vyhled\u00E1v\u00E1ny mutace, kter\u00E9 potla\u010Duj\u00ED p\u016Fvodn\u00ED agravitropn\u00ED fenotypov\u00FD projev t\u00E9to transgenn\u00ED linie a v populaci PIN1-GFP s fluorescen\u010Dn\u011B zna\u010Den\u00FDm PIN1 proteinem, byly vyhled\u00E1v\u00E1ny mutace kter\u00E9 negativn\u011B ovliv\u0148uj\u00ED sm\u011Brov\u00E1n\u00ED PIN1-GFP na plazmatickou membr\u00E1nu."@cs . . . . . "[4E9A5232EE8D]" . "\u010Cesk\u00E1 spole\u010Dnost experiment\u00E1ln\u00ED biologie rostlin" . "Zemov\u00E1, Radka" . . "4"^^ . "Identifikace a pozi\u010Dn\u00ED klonov\u00E1n\u00ED komponent auxinov\u00E9ho transportu a bun\u011B\u010Dn\u00E9 polarity u Arabidopsis thaliana"@cs . "Identification and positional cloning of components of auxin transport and cell polarity in Arabidopsis thaliana"@en . "P(LC06034), Z(MSM0021622415)" . "1213-6670" . . . "Hej\u00E1tko, Jan" . "4"^^ . . . "Development of plant body strongly depends on intercellular transport and distribution of hormone auxin. Mechanism of polar auxin transport has remained elusive despite considerable efforts towards the biochemical characterization of binding sites and several screens for auxin-transport-inhibitor mutants. Using a fungal toxin Brefeldin A, which inhibits recycling of proteins in cells, it has been shown that Arabidopsis auxin transport proteins from the PIN family constitutively cycles between the plasma membrane and endosomal compartments. In situ visualization of putative auxin efflux carriers PIN1 and PIN2 revealed their asymmetric distribution in the plasma membrane that correlates with direction of auxin flow. Using forward genetic we have screened for EMS-induced mutants with changed PIN activity and cellular localization. We identified several mutants affected in these processes and recently we are working on identification of affected genes via positional cloning of the corresponding mutations."@en . "Identifikace a pozi\u010Dn\u00ED klonov\u00E1n\u00ED komponent auxinov\u00E9ho transportu a bun\u011B\u010Dn\u00E9 polarity u Arabidopsis thaliana"@cs . "Kov\u00E1\u0159ov\u00E1, Pavl\u00EDna" . . . "Identifikace a pozi\u010Dn\u00ED klonov\u00E1n\u00ED komponent auxinov\u00E9ho transportu a bun\u011B\u010Dn\u00E9 polarity u Arabidopsis thaliana" . "1"^^ . . . "Identification and positional cloning of components of auxin transport and cell polarity in Arabidopsis thaliana"@en . "Arabidopsis thaliana; hormone auxin"@en . . . "RIV/00216224:14310/07:00024018" . "Friml, Ji\u0159\u00ED" . "Olomouc" . "14310" . "Olomouc" . . . "425327" . . "2008-07-09+02:00"^^ .