"RIV/00216224:14310/07:00022495!RIV10-MZE-14310___" . "2"^^ . "978-80-210-4234-6" . . "2007-01-31+01:00"^^ . . . "\u0160panov\u00E1, Alena" . "P(QF3169), Z(MSM0021622415)" . . . "XI. Setk\u00E1n\u00ED biochemik\u016F a molekul\u00E1rn\u00EDch biolog\u016F" . "Bifidobacterium; ARDRA; identification"@en . . "Vyu\u017Eit\u00ED metody ARDRA pro druhovou identifikaci nov\u00FDch kmen\u016F bakteri\u00ED rodu Bifidobacterium" . . . "RIV/00216224:14310/07:00022495" . "Brno" . "14310" . "Brno" . . "ARDRA analysis was used for the identification of 11 selected bacterial strains isolated from human sources. These strains were identified into the genus Bifidobacterium using genus-specific PCR. However they could not be identified into species using species-specific PCR due to the cross reactions. Analysis of genus specific amplicon of 914 bp using restrictases AluI and Sau3AI enabled the differentiation of species B. adolescentis, B. bifidum, B. breve, B. catenulatum, B. infantis and B. longum. Alltogether 10 strains could be identified into species. In conclusion, ARDRA analysis is a quick and reproducible identification procedure with high discrimination potential for individual species."@en . "Masarykova univerzita" . . "Vyu\u017Eit\u00ED metody ARDRA pro druhovou identifikaci nov\u00FDch kmen\u016F bakteri\u00ED rodu Bifidobacterium"@cs . "Pomoc\u00ED ARDRA anal\u00FDzy identifikovat bakteri\u00E1ln\u00ED kmeny isolovan\u00E9 z hum\u00E1nn\u00EDch zdroj\u016F, kter\u00E9 byly rodov\u011B specifickou PCR za\u0159azeny do rodu Bifidobacterium a kter\u00E9 p\u0159i pou\u017Eit\u00ED druhov\u011B specifick\u00FDch PCR vykazovaly zk\u0159\u00ED\u017Een\u00E9 reakce a proto nemohly b\u00FDt za\u0159azeny do druh\u016F. Restrik\u010Dn\u00ED anal\u00FDza amplikonu 914bp pomoc\u00ED restrikt\u00E1z AluI a Sau3 AI umo\u017Enila odli\u0161en\u00ED druh\u016F B .adolescentis, B. bifidum, B. breve, B. catenulatum, B. infantis a B. longum. Celkem bylo rozli\u0161eno a za\u0159azeno do druh\u016F deset z jeden\u00E1cti nov\u011B isolovan\u00FDch kmen\u016F. Lze konstatovat, \u017Ee ARDRA anal\u00FDza je rychl\u00E1 a reprodukovateln\u00E1 identifika\u010Dn\u00ED metoda s vysok\u00FDm diskrimina\u010Dn\u00EDm potenci\u00E1lem na \u00FArovni druhu."@cs . "Vyu\u017Eit\u00ED metody ARDRA pro druhovou identifikaci nov\u00FDch kmen\u016F bakteri\u00ED rodu Bifidobacterium" . . "The use of ARDRA method for specific identification of new Bifidobacterium strains"@en . . . . "460175" . "K\u0161icov\u00E1, Kate\u0159ina" . "3"^^ . . "[E2D4896A1B57]" . . "3"^^ . "Vyu\u017Eit\u00ED metody ARDRA pro druhovou identifikaci nov\u00FDch kmen\u016F bakteri\u00ED rodu Bifidobacterium"@cs . "Rittich, Bohuslav" . . . "Pomoc\u00ED ARDRA anal\u00FDzy identifikovat bakteri\u00E1ln\u00ED kmeny isolovan\u00E9 z hum\u00E1nn\u00EDch zdroj\u016F, kter\u00E9 byly rodov\u011B specifickou PCR za\u0159azeny do rodu Bifidobacterium a kter\u00E9 p\u0159i pou\u017Eit\u00ED druhov\u011B specifick\u00FDch PCR vykazovaly zk\u0159\u00ED\u017Een\u00E9 reakce a proto nemohly b\u00FDt za\u0159azeny do druh\u016F. Restrik\u010Dn\u00ED anal\u00FDza amplikonu 914bp pomoc\u00ED restrikt\u00E1z AluI a Sau3 AI umo\u017Enila odli\u0161en\u00ED druh\u016F B .adolescentis, B. bifidum, B. breve, B. catenulatum, B. infantis a B. longum. Celkem bylo rozli\u0161eno a za\u0159azeno do druh\u016F deset z jeden\u00E1cti nov\u011B isolovan\u00FDch kmen\u016F. Lze konstatovat, \u017Ee ARDRA anal\u00FDza je rychl\u00E1 a reprodukovateln\u00E1 identifika\u010Dn\u00ED metoda s vysok\u00FDm diskrimina\u010Dn\u00EDm potenci\u00E1lem na \u00FArovni druhu." . . . "The use of ARDRA method for specific identification of new Bifidobacterium strains"@en .