. "978-80-89088-51-5" . "Star\u00E1 Lesn\u00E1" . . "Star\u00E1 Lesn\u00E1" . "Molecular analysis of Frigida (Fri) gene in late-flowering genotypes of Arabidopsis thaliana"@en . "434830" . . . "Z(MSM0021622415)" . . . . "L\u00EDzal, Pavel" . "\u00DAstav genetiky a biotechnol\u00F3gi\u00ED rastl\u00EDn SAV" . "Flowering time is stimulated by both environmental (light, temperature) and endogenous signals. FLC and FRI genes are the most important endogenous signals controlling onset of flowering in natural populations. FLC is a significant flowering repressor under the control of the FRI gene. Interaction of both genes is the reason of lateness in natural populations. In the FRI gene, two frequent deletions responsible for early-flowering phenotype, Ler- and Col-type are known. These deletions have been identified only in early-flowering plants but never in late-flowering plants. Therefore, our work was focused on molecular analysis of FRI gene in late-flowering ecotypes derived from natural populations in the Czech Republic (Je-4, Je-18, Je-27, Je-28 and Hod) and in four late-flowering mutants (dn, L4, Spi and M73). For this purpose five PCR primers that cover the complete sequence of the FRI gene including the promoter were used. Deletions or insertions were detected by agarose or polyacrylamide gels." . . "2"^^ . . "4"^^ . "Flowering time is stimulated by both environmental (light, temperature) and endogenous signals. FLC and FRI genes are the most important endogenous signals controlling onset of flowering in natural populations. FLC is a significant flowering repressor under the control of the FRI gene. Interaction of both genes is the reason of lateness in natural populations. In the FRI gene, two frequent deletions responsible for early-flowering phenotype, Ler- and Col-type are known. These deletions have been identified only in early-flowering plants but never in late-flowering plants. Therefore, our work was focused on molecular analysis of FRI gene in late-flowering ecotypes derived from natural populations in the Czech Republic (Je-4, Je-18, Je-27, Je-28 and Hod) and in four late-flowering mutants (dn, L4, Spi and M73). For this purpose five PCR primers that cover the complete sequence of the FRI gene including the promoter were used. Deletions or insertions were detected by agarose or polyacrylamide gels."@en . "4"^^ . . "[F0218E1F5810]" . "Cetkovsk\u00E1, Kate\u0159ina" . . "RIV/00216224:14310/07:00022293" . . "14310" . "Molecular analysis of Frigida (Fri) gene in late-flowering genotypes of Arabidopsis thaliana" . "Plant biotechnology: impact on high quality plant reproduction" . "Molecular analysis of Frigida (Fri) gene in late-flowering genotypes of Arabidopsis thaliana"@en . "RIV/00216224:14310/07:00022293!RIV11-MSM-14310___" . . . . . "Arabidopsis thaliana; flowering time; mutation; RT-PCR"@en . . . . . "2007-01-01+01:00"^^ . "\u0158epkov\u00E1, Jana" . "Relichov\u00E1, Ji\u0159ina" . "Molecular analysis of Frigida (Fri) gene in late-flowering genotypes of Arabidopsis thaliana" .