"AhR-mediated and antiestrogenic activity of humic substances" . . "Hilscherov\u00E1, Kl\u00E1ra" . . "Holoubek, Ivan" . . "RIV/00216224:14310/07:00020344" . . "Bl\u00E1ha, Lud\u011Bk" . . "000245846700005" . "Jano\u0161ek, Jaroslav" . "6"^^ . "0045-6535" . "P(GP525/05/P160), Z(MSM0021622412)" . "14310" . "[D855070859D3]" . . . "6"^^ . "Humic substances (HS) were for decades regarded as inert in the ecosystems with respect to their possible toxicity. However, HS have been recently shown to elicit various adverse effects generally attributed to xenobiotics. In our study, we used MVLN and H4IIE-luc cell lines stably transfected with luciferase gene under control of estrogen receptor (ER) and Ah receptor (AhR; receptor connected with so-called dioxin-like toxicity) for assessment of anti/estrogenic and AhR-mediated effects of 12 commercially available humic substances. Out of those, five humic acids were shown to induce AhR-mediated activity with relative potencies related to TCDD 2.6 x 10-8-7.4 x 10-8. Organic extracts of HS solutions also elicited high activities what means that lipophilic molecules are responsible for a great part of effect. However, relatively high activity remaining in extracted solution suggests also presence of polar AhR-agonists."@en . . . . . . "408796" . "5"^^ . "Chemosphere" . . "67" . "RIV/00216224:14310/07:00020344!RIV10-MSM-14310___" . "Giesy, J. P." . "6" . "Ah receptor; AhR-mediated activity; Antiestrogenic effects; Endocrine disruption"@en . . "CZ - \u010Cesk\u00E1 republika" . . "AhR-mediated and antiestrogenic activity of humic substances"@en . . . "Bittner, Michal" . . "AhR-mediated and antiestrogenic activity of humic substances" . "AhR-mediated and antiestrogenic activity of humic substances"@en . . "Humic substances (HS) were for decades regarded as inert in the ecosystems with respect to their possible toxicity. However, HS have been recently shown to elicit various adverse effects generally attributed to xenobiotics. In our study, we used MVLN and H4IIE-luc cell lines stably transfected with luciferase gene under control of estrogen receptor (ER) and Ah receptor (AhR; receptor connected with so-called dioxin-like toxicity) for assessment of anti/estrogenic and AhR-mediated effects of 12 commercially available humic substances. Out of those, five humic acids were shown to induce AhR-mediated activity with relative potencies related to TCDD 2.6 x 10-8-7.4 x 10-8. Organic extracts of HS solutions also elicited high activities what means that lipophilic molecules are responsible for a great part of effect. However, relatively high activity remaining in extracted solution suggests also presence of polar AhR-agonists." . . . .