. . "Bene\u0161, Petr" . . "A proteomic analysis of protein variations during differentiation of v-myb-transformed monoblasts"@en . "RIV/00216224:14310/07:00020085" . "31" . . "P(GA301/03/1055), P(GA301/06/0036), P(GP301/03/D022), Z(MSM0021622415)" . . . . . . "A proteomic analysis of protein variations during differentiation of v-myb-transformed monoblasts" . "14310" . . "Ondrou\u0161kov\u00E1, Eva" . . "Kone\u010Dn\u00E1, Hana" . . "A proteomic analysis of protein variations during differentiation of v-myb-transformed monoblasts"@en . . . . "\u0160marda, Jan" . . . . "0145-2126" . "V\u00E1\u0148a, Petr" . "\u010Cern\u00E1, Karol\u00EDna" . "Zdr\u00E1hal, Zbyn\u011Bk" . . "v-myb; monoblast; macrophage; differentiation; proteome; two-dimensional gel electrophoresis; trichostatin A; TPA; mass spectrometry"@en . . . "7"^^ . "[3175217B7FCA]" . "v-myb-oncogene of avian myeloblastosis virus transforms myelomonocytic cells in vitro and induces acute monoblastic leukemia in vivo. The transforming effect of the v-myb can be suppressed using phorbol ester (TPA) or histone deacetylase inhibitor trichostatin A (TSA), the inducers of cell differentiation that are in clinical trials. In this study, we used proteomics-based approach to identify proteins with variable expression in differentiated BM2 cells. Proteome variations induced by TPA and TSA were compared to examine the mechanism of differentiation-promoting effects of these drugs. We found that expression of several proteins participating in cell cytoskeleton rearrangement, heat shock response, proteosynthesis and cell signaling was altered in TPA- or TSA-treated cells. We present here the first comparative proteome analysis of v-myb-transformed monoblasts BM2 focused on identification of proteins involved in their terminal differentiation."@en . . . "7"^^ . . . "Leukemia Research" . . . "9"^^ . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "RIV/00216224:14310/07:00020085!RIV10-MSM-14310___" . . "v-myb-oncogene of avian myeloblastosis virus transforms myelomonocytic cells in vitro and induces acute monoblastic leukemia in vivo. The transforming effect of the v-myb can be suppressed using phorbol ester (TPA) or histone deacetylase inhibitor trichostatin A (TSA), the inducers of cell differentiation that are in clinical trials. In this study, we used proteomics-based approach to identify proteins with variable expression in differentiated BM2 cells. Proteome variations induced by TPA and TSA were compared to examine the mechanism of differentiation-promoting effects of these drugs. We found that expression of several proteins participating in cell cytoskeleton rearrangement, heat shock response, proteosynthesis and cell signaling was altered in TPA- or TSA-treated cells. We present here the first comparative proteome analysis of v-myb-transformed monoblasts BM2 focused on identification of proteins involved in their terminal differentiation." . . "A proteomic analysis of protein variations during differentiation of v-myb-transformed monoblasts" . "2" . "408122" . .