"4"^^ . "RIV/00216224:14310/06:00017828" . "RIV/00216224:14310/06:00017828!RIV10-MSM-14310___" . "The use of DNA analysis permits to resolve many taxonomic and phylogenetic problems at different levels. In this study, we try to give a summary of used genetic markers on different taxonomic levels in the phylogenetic study of Diptera and a methodical overview of the laboratory methods. The gene choice is of crucial importance. Some of the gene markers are applicable in a wide range of taxonomic levels in the Diptera flies (16S, COI, 18S, tRNA-leucine), other are limited to lower (subgeneric or intraspecific) level (cytB). Within the gene choice, the proper DNA extraction is also very important task, what works upon a success of the whole process."@en . "Diptera molecular markers phylogeny"@en . "4"^^ . "9"^^ . . "486620" . "Acta Universitatis Carolinae" . . "Molecular markers used in phylogenetic studies of Diptera with a methodological overview"@en . "Va\u0148hara, Jarom\u00EDr" . . "1-2" . . "Bejd\u00E1k, Petr" . "Molecular markers used in phylogenetic studies of Diptera with a methodological overview" . "0001-7132" . "[26EB81F8C597]" . . "Molecular markers used in phylogenetic studies of Diptera with a methodological overview" . . . "Molecular markers used in phylogenetic studies of Diptera with a methodological overview"@en . . "CZ - \u010Cesk\u00E1 republika" . "50" . . "Z(MSM0021622416)" . . "T\u00F3thov\u00E1, Andrea" . "Bryja, Josef" . "14310" . . . . . . "The use of DNA analysis permits to resolve many taxonomic and phylogenetic problems at different levels. In this study, we try to give a summary of used genetic markers on different taxonomic levels in the phylogenetic study of Diptera and a methodical overview of the laboratory methods. The gene choice is of crucial importance. Some of the gene markers are applicable in a wide range of taxonomic levels in the Diptera flies (16S, COI, 18S, tRNA-leucine), other are limited to lower (subgeneric or intraspecific) level (cytB). Within the gene choice, the proper DNA extraction is also very important task, what works upon a success of the whole process." . .