"373" . . . . "[8F68E6D61DA5]" . "0009-2770" . . "P(GD204/03/H016), Z(MSM0021622413)" . . "Mutageneze lektinu PA-IIL a souvisejici zm\u011Bny v afinitn\u00EDm chov\u00E1ni v\u016F\u010Di sacharid\u016Fm - experiment\u00E1lni a vypo\u010Detn\u00ED studie"@cs . . . "PA-IIL lectin mutagenesis and the study of affinity changes bymeans of experimental and computational chemistry"@en . "Imberty, Anne" . . "In order to characterise the contribution of particular amino acids for specificity, mutants of PA-IIL concerning Ser22, Ser23 and Gly24 that are responsible for the high preference of PA-IIL to fucose have been designed. Mutants have been prepared by site-directed mutagenesis and purified by affinity chromatography on mannose-agarose column. Interaction of mutants with different monosaccharides has been evaluated using isothermal titration microcalorimetry and some complexes have been crystallised. Experiments showed the importance of amino acid residue on position 22 and opened the feasibility for prediction of specificity for the other PA-IIL-like lectins. Simultaneously, the program TRITON, a graphical tool for modelling protein mutants, which has been under development in our laboratory, was used for designing protein mutants in silico. TRITON was used to create one-, two- and three-point mutants of PA-IIL at the 22, 23 and 24 positions. These mutants were used for docking experiments with a numb"@en . . . "100" . "Adam, Jan" . "Chemick\u00E9 listy" . "RIV/00216224:14310/06:00015930" . "PA-IIL lectin mutagenesis and the study of affinity changes bymeans of experimental and computational chemistry"@en . . "5" . "Charakterizace p\u0159\u00EDsp\u011Bvku jednotliv\u00FDch aminokyselin lektinu PA-IIL pro afinitu a specifitu k sacharidov\u00FDm ligand\u016Fm byla prov\u00E1d\u011Bna mutagenezi in silico a in vitro zm\u00EDn\u011Bn\u00EDho lektinu a charakterizac\u00ED pomoc\u00ED ITC a v\u00FDpo\u010Detn\u00EDch metod." . "14310" . "Charakterizace p\u0159\u00EDsp\u011Bvku jednotliv\u00FDch aminokyselin lektinu PA-IIL pro afinitu a specifitu k sacharidov\u00FDm ligand\u016Fm byla prov\u00E1d\u011Bna mutagenezi in silico a in vitro zm\u00EDn\u011Bn\u00EDho lektinu a charakterizac\u00ED pomoc\u00ED ITC a v\u00FDpo\u010Detn\u00EDch metod."@cs . . "487375" . "K\u0159\u00ED\u017E, Zden\u011Bk" . . "Ko\u010Da, Jaroslav" . "Mutageneze lektinu PA-IIL a souvisejici zm\u011Bny v afinitn\u00EDm chov\u00E1ni v\u016F\u010Di sacharid\u016Fm - experiment\u00E1lni a vypo\u010Detn\u00ED studie" . "RIV/00216224:14310/06:00015930!RIV08-MSM-14310___" . . . . "Mutageneze lektinu PA-IIL a souvisejici zm\u011Bny v afinitn\u00EDm chov\u00E1ni v\u016F\u010Di sacharid\u016Fm - experiment\u00E1lni a vypo\u010Detn\u00ED studie"@cs . "lectin; microcalorimetry; mutagenesis; pathogen"@en . . "Prokop, Martin" . . . "7"^^ . "Wimmerov\u00E1, Michaela" . "6"^^ . "Mutageneze lektinu PA-IIL a souvisejici zm\u011Bny v afinitn\u00EDm chov\u00E1ni v\u016F\u010Di sacharid\u016Fm - experiment\u00E1lni a vypo\u010Detn\u00ED studie" . "1"^^ . . "CZ - \u010Cesk\u00E1 republika" . . . . "Pokorn\u00E1, Martina" .