. "Hernychov\u00E1, Lenka" . "Bacteriophage 812 is a polyvalent phage with very broad host range in the genus Staphylococcus, which makes it a suitable candidate for phage therapy of staphylococcal infections. The genomes of polyvalent staphylococcal phages exceed 125 kbp and include about 200 putative open reading frames. Our study was focused on characterization of the phage proteome, especially on identification of phage virion proteins. Combination of both one- and two-dimensional electrophoreses (1-DE and 2-DE) followed by peptide mass fingerprinting using MALDI-TOF mass spectrometry, led to the identification of 24 virion proteins of the phage 812. Twenty of them were not identified by proteome analysis of closely related staphylococcal phages K and G1 yet. Although the phage proteome is much simpler compared to that of cellular organisms, using 2-DE in addition to 1-DE significantly increased the number of identified proteins." . . . . . . "Bacteriophage 812 is a polyvalent phage with very broad host range in the genus Staphylococcus, which makes it a suitable candidate for phage therapy of staphylococcal infections. The genomes of polyvalent staphylococcal phages exceed 125 kbp and include about 200 putative open reading frames. Our study was focused on characterization of the phage proteome, especially on identification of phage virion proteins. Combination of both one- and two-dimensional electrophoreses (1-DE and 2-DE) followed by peptide mass fingerprinting using MALDI-TOF mass spectrometry, led to the identification of 24 virion proteins of the phage 812. Twenty of them were not identified by proteome analysis of closely related staphylococcal phages K and G1 yet. Although the phage proteome is much simpler compared to that of cellular organisms, using 2-DE in addition to 1-DE significantly increased the number of identified proteins."@en . "P(GA203/03/0515), Z(MSM0021622415)" . . "RIV/00216224:14310/06:00015836" . "RIV/00216224:14310/06:00015836!RIV10-MSM-14310___" . "Do\u0161ka\u0159, Ji\u0159\u00ED" . . . . . "Proteomic characterization of the polyvalent staphylophage 812"@en . . . . . "Preisler, Jan" . . "14310" . "Zdr\u00E1hal, Zbyn\u011Bk" . "Kone\u010Dn\u00E1, Hana" . "Proteomic characterization of the polyvalent staphylophage 812" . "Eyer, Lud\u011Bk" . . . "[A7D85661D03C]" . . . "8"^^ . . "495704" . "Proteomic characterization of the polyvalent staphylophage 812"@en . "8"^^ . "R\u016F\u017Ei\u010Dkov\u00E1, Vladislava" . "Staphylococcus aureus; medical microbiology; molecular diagnostics; phage therapy; proteomics"@en . . . . . . "Proteomic characterization of the polyvalent staphylophage 812" . "Pant\u016F\u010Dek, Roman" .