. "2"^^ . . "98" . "2"^^ . "3" . . . . . "Intraspecific DNA content variability in Festuca pallens on different geographical scales and ploidy levels"@en . "0305-7364" . "000239898800022" . "Annals of Botany" . . "480270" . . "Intraspecific DNA content variability in Festuca pallens on different geographical scales and ploidy levels"@en . . . . "RIV/00216224:14310/06:00015792!RIV10-MSM-14310___" . . . . . "Background and aims: Intraspecific genome size variability of Festuca pallens Host (Poaceae, Poaeae) occurring on relict rocky steppes in Central Europe was studied on two ploidy levels and three geographical scales: (i) local scale of 24 populations; 3 to 6 plants per population; (ii) landscape scale of three 9 to 13 km long transects in river canyons or hill systems; 15 to 34 samples per transect; (iii) global scale of 160 samples covering the whole distribution area. Methods: DAPI flow cytometry; homogenously cultivated samples (more than 1 year); measured randomly during one week with two internal standards Lycopersicon esculentum, Pisum sativum. The differences in DNA content were confirmed by the double-peaks of simultaneously measured samples. Key results: On a global scale, the DNA content ranged 1.170 fold in diploids, and 1.164 fold in tetraploids. In 16 of 24 populations significant variability was proved (P<0.001). On a landscape scale, a maximum difference of 1." . . "P(GA206/03/0228), P(LC06073), Z(MSM0021622416)" . . . "14"^^ . "Intraspecific DNA content variability in Festuca pallens on different geographical scales and ploidy levels" . "Festuca subg. Festuca; fescue; Gramineae; polyploidy; Poaceae; genome size; flow cytometry; phytogeography; Central Europe; infraspecific C-value variation; DAPI"@en . . "Background and aims: Intraspecific genome size variability of Festuca pallens Host (Poaceae, Poaeae) occurring on relict rocky steppes in Central Europe was studied on two ploidy levels and three geographical scales: (i) local scale of 24 populations; 3 to 6 plants per population; (ii) landscape scale of three 9 to 13 km long transects in river canyons or hill systems; 15 to 34 samples per transect; (iii) global scale of 160 samples covering the whole distribution area. Methods: DAPI flow cytometry; homogenously cultivated samples (more than 1 year); measured randomly during one week with two internal standards Lycopersicon esculentum, Pisum sativum. The differences in DNA content were confirmed by the double-peaks of simultaneously measured samples. Key results: On a global scale, the DNA content ranged 1.170 fold in diploids, and 1.164 fold in tetraploids. In 16 of 24 populations significant variability was proved (P<0.001). On a landscape scale, a maximum difference of 1."@en . . . . . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "RIV/00216224:14310/06:00015792" . . "\u0160marda, Petr" . "[9DE6B69BF676]" . "Bure\u0161, Petr" . "Intraspecific DNA content variability in Festuca pallens on different geographical scales and ploidy levels" . "14310" . .