"468279" . . . . "Change of the Protein p53 Electrochemical Signal According to its Structural Form - Quick and Sensitive Distinguishing of Native, Denatured, and Aggregated Form of the %22Guardian of the Genome%22" . "25" . . "Change of the Protein p53 Electrochemical Signal According to its Structural Form - Quick and Sensitive Distinguishing of Native, Denatured, and Aggregated Form of the %22Guardian of the Genome%22"@en . "Mikelov\u00E1, Radka" . "[B8D364DCA673]" . "10"^^ . "Pot\u011B\u0161il, David" . . . . "p53; .ow injection analysis; electrochemical detection; structural changes; carbon"@en . "RIV/00216224:14310/06:00015697!RIV10-GA0-14310___" . "CZ - \u010Cesk\u00E1 republika" . . . "1572-3887" . . . . "Kizek, Ren\u00E9" . . "1" . . "Presence of mutated and/or structurally modi.ed (e.g., denatured, aggregated) protein p53 form is associated with several disorders such as Alzheimers disease, Parkinsons disease, prion diseases, and many types of tumours. The aim of this work was to distinguish native, denatured and aggregated form of full-length p53 by .ow injection analysis coupled with electrochemical detector (FIA-ED). Firstly FIA-ED method used for protein native form determination was optimized (detection limit 45.8 amol per 5 ll injection; 3_S/N). In addition the technique was applied to identify p53 structural forms (denatured and aggregated). It was found out that denatured form provides about three times higher electrochemical response (protein structure unfolding, approach of more electroactive centers - aminoacid residues - towards electrode surface) in comparison with native form. On the other hand, aggregated form o." . . "Change of the Protein p53 Electrochemical Signal According to its Structural Form - Quick and Sensitive Distinguishing of Native, Denatured, and Aggregated Form of the %22Guardian of the Genome%22"@en . "14310" . "P(GP525/04/P132)" . . "Presence of mutated and/or structurally modi.ed (e.g., denatured, aggregated) protein p53 form is associated with several disorders such as Alzheimers disease, Parkinsons disease, prion diseases, and many types of tumours. The aim of this work was to distinguish native, denatured and aggregated form of full-length p53 by .ow injection analysis coupled with electrochemical detector (FIA-ED). Firstly FIA-ED method used for protein native form determination was optimized (detection limit 45.8 amol per 5 ll injection; 3_S/N). In addition the technique was applied to identify p53 structural forms (denatured and aggregated). It was found out that denatured form provides about three times higher electrochemical response (protein structure unfolding, approach of more electroactive centers - aminoacid residues - towards electrode surface) in comparison with native form. On the other hand, aggregated form o."@en . "5"^^ . . "RIV/00216224:14310/06:00015697" . . . "The Protein Journal" . "Change of the Protein p53 Electrochemical Signal According to its Structural Form - Quick and Sensitive Distinguishing of Native, Denatured, and Aggregated Form of the %22Guardian of the Genome%22" . "Adam, Vojt\u011Bch" . "4"^^ . . "Pr\u016F\u0161a, Richard" .