"Z(MSM0021622416)" . "Swings, Jean" . "2"^^ . "Enterococcus; (GTG)5-PCR fingerprinting; bryndza"@en . "Seman, Milan" . "Lefebvre, Karen" . "14310" . "Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese" . "Sedl\u00E1\u010Dek, Ivo" . "RIV/00216224:14310/05:00013899" . "\u0160vec, Pavel" . . "RIV/00216224:14310/05:00013899!RIV10-MSM-14310___" . "7"^^ . "[CFAE1FA0D980]" . . . . . . "A group of previously well-identified enterococci isolated from a typical Slovakian sheep-milk product bryndza were characterized by the (GTG)5-PCR fingerprinting in order to evaluate discriminatory power of this method for identification and strain-typing. Analysed group consisted of E. faecium (n = 76), E. faecalis (n = 25) and E. durans (n = 10) strains. They were sampled from two bryndza-processing manufactories during three monthly samplings performed successively (April, May and June) in 2000 and 2001. Isolated strains were characterized and identified by biotyping, ITS-PCR, ddlE.faecalis and ddlE.faecium PCRs. Species identity of representative strains selected from each (GTG)5-PCR fingerprint subcluster was confirmed by SDS-PAGE of whole-cell proteins. The (GTG)5-PCR fingerprint profiles obtained from E. faecalis strains were visually very close. Similarly, E. durans strains revealed nearly identical fingerprints. E."@en . "A group of previously well-identified enterococci isolated from a typical Slovakian sheep-milk product bryndza were characterized by the (GTG)5-PCR fingerprinting in order to evaluate discriminatory power of this method for identification and strain-typing. Analysed group consisted of E. faecium (n = 76), E. faecalis (n = 25) and E. durans (n = 10) strains. They were sampled from two bryndza-processing manufactories during three monthly samplings performed successively (April, May and June) in 2000 and 2001. Isolated strains were characterized and identified by biotyping, ITS-PCR, ddlE.faecalis and ddlE.faecium PCRs. Species identity of representative strains selected from each (GTG)5-PCR fingerprint subcluster was confirmed by SDS-PAGE of whole-cell proteins. The (GTG)5-PCR fingerprint profiles obtained from E. faecalis strains were visually very close. Similarly, E. durans strains revealed nearly identical fingerprints. E." . "Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese"@en . "Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese"@en . . . "Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese" . "520723" . . "Snauwaert, Cindy" . . . . . "Vancanneyt, Marc" . . . .