"4"^^ . . "99" . . . . "Zdr\u00E1hal, Zbyn\u011Bk" . . "5"^^ . "Charakterizace proteomu bakteriof\u00E1ga 812" . "Lytick\u00FD bakteriof\u00E1g 812 pat\u0159\u00ED mezi slibn\u00E9 kandid\u00E1ty na terapii stafylokokov\u00FDch infekc\u00ED jako n\u00E1hrada l\u00E9\u010Dby antibiotiky. Proteom bakteriof\u00E1ga 812 byl analyzov\u00E1n vybran\u00FDmi technikami hmotnostn\u00ED spektrometrie a separace. Bylo identifikov\u00E1no jeden\u00E1ct protein\u016F z toho sedm poprv\u00E9. Gelov\u00E1 elektrofor\u00E9za s peptidov\u00FDm mapov\u00E1n\u00EDm byla shled\u00E1na nej\u00FA\u010Dinn\u011Bj\u0161\u00ED technikou pro celkovou anal\u00FDzu; HPLC-ESI MS byla pou\u017Eita pro potvrzen\u00ED ve sporn\u00FDch p\u0159\u00EDpadech. Zd\u00E1nliv\u011B anom\u00E1ln\u00ED migrace hlavn\u00EDho proteinu bi\u010D\u00EDkov\u00E9 pochvy (812_mtsp) byla vysv\u011Btlena po separaci gelovou elektrofor\u00E9zou za upraven\u00FDch podm\u00EDnek a peptidov\u00FDm mapov\u00E1n\u00EDm pomoc\u00ED MALDI TOF a TOF/TOF MS. T\u0159i fragmenty proteinu 812_mtsp o podobn\u00E9 velikosti, kter\u00E9 p\u016Fvodn\u011B migrovaly v jedn\u00E9 z\u00F3n\u011B, byly odd\u011Bleny a identifikov\u00E1ny. Charakterizace proteomu bakteriof\u00E1ga 812 je p\u0159edpokladem pro budouc\u00ED srovn\u00E1n\u00ED standardn\u00EDho f\u00E1ga 812 s jeho mutanty a p\u0159\u00EDbuzn\u00FDmi f\u00E1gy a korelaci zm\u011Bn proteomu se zm\u011Bnami rozmez\u00ED hostitele." . "12" . . "Charakterization of bacteriphage 812 proteome"@en . . . "CZ - \u010Cesk\u00E1 republika" . "Preisler, Jan" . "P(GA203/03/0515), Z(MSM0021622415)" . . "RIV/00216224:14310/05:00012894" . . . "bakteriophage; proteome; mass spectrometry; gel electrophoresis; anomal migration"@en . "Charakterizace proteomu bakteriof\u00E1ga 812" . . "000234182000014" . "Charakterizace proteomu bakteriof\u00E1ga 812"@cs . "515098" . . "Charakterization of bacteriphage 812 proteome"@en . "[778D1B0D8FFF]" . "Chemick\u00E9 listy" . "14310" . "Charakterizace proteomu bakteriof\u00E1ga 812"@cs . . . "A lytic bacteriophage 812 ranks among to promising candidates for phage therapy, which emerges as an alternative to antibiotics for treatment of staphylococcal infections. Proteome of bacteriophage 812 was analyzed using various mass spectrometry techniques. Eleven proteins were identified, of which seven proteins were identified for the first time. Gel electrophoresis with peptide mass fingerprinting was found the most efficient for overall analysis; HPLC-ESI MS was used for confirmation in dubious cases. Seemingly abnormal migration of main tail sheath protein (812_mtsp) was explained after adjustment of gel electrophoresis conditions and peptide mass fingerprinting combined with MALDI TOF/TOF MS results. Three similarly-sized 812_mtsp fragments, which originally migrated in one band, were separated and identified."@en . . "Eyer, Lud\u011Bk" . "4"^^ . . . . . . . "Kone\u010Dn\u00E1, Hana" . "Lytick\u00FD bakteriof\u00E1g 812 pat\u0159\u00ED mezi slibn\u00E9 kandid\u00E1ty na terapii stafylokokov\u00FDch infekc\u00ED jako n\u00E1hrada l\u00E9\u010Dby antibiotiky. Proteom bakteriof\u00E1ga 812 byl analyzov\u00E1n vybran\u00FDmi technikami hmotnostn\u00ED spektrometrie a separace. Bylo identifikov\u00E1no jeden\u00E1ct protein\u016F z toho sedm poprv\u00E9. Gelov\u00E1 elektrofor\u00E9za s peptidov\u00FDm mapov\u00E1n\u00EDm byla shled\u00E1na nej\u00FA\u010Dinn\u011Bj\u0161\u00ED technikou pro celkovou anal\u00FDzu; HPLC-ESI MS byla pou\u017Eita pro potvrzen\u00ED ve sporn\u00FDch p\u0159\u00EDpadech. Zd\u00E1nliv\u011B anom\u00E1ln\u00ED migrace hlavn\u00EDho proteinu bi\u010D\u00EDkov\u00E9 pochvy (812_mtsp) byla vysv\u011Btlena po separaci gelovou elektrofor\u00E9zou za upraven\u00FDch podm\u00EDnek a peptidov\u00FDm mapov\u00E1n\u00EDm pomoc\u00ED MALDI TOF a TOF/TOF MS. T\u0159i fragmenty proteinu 812_mtsp o podobn\u00E9 velikosti, kter\u00E9 p\u016Fvodn\u011B migrovaly v jedn\u00E9 z\u00F3n\u011B, byly odd\u011Bleny a identifikov\u00E1ny. Charakterizace proteomu bakteriof\u00E1ga 812 je p\u0159edpokladem pro budouc\u00ED srovn\u00E1n\u00ED standardn\u00EDho f\u00E1ga 812 s jeho mutanty a p\u0159\u00EDbuzn\u00FDmi f\u00E1gy a korelaci zm\u011Bn proteomu se zm\u011Bnami rozmez\u00ED hostitele."@cs . "0009-2770" . "RIV/00216224:14310/05:00012894!RIV10-MSM-14310___" .