. . "8"^^ . . "Virulent and polyvalent staphylophage 812, a member of Myoviridae family, exhibits strong lytic activity on a broad host range of Staphylococcus species, thus being a suitable candidate for treatment of staphylococcal infections. Genome of this phage is represented by linear 145 kb dsDNA. Our study was focused on genome and proteome comparison of 812 wild-type phage, its host-range mutants and related phages U16, 131 and SK311 with the aim to explain molecular basis of differences in their host-range. Comparison of the phage genomes revealed several rearrangements of DNA sequences as well as single nucleotide substitutions in different parts of the genomes. Nucleotide insertions and deletions of various size (~1 to 3 kb) were most frequently found in non-coding terminal genomic regions in phages with different host range. We tried to find possible correlations between mutational changes on DNA and proteome composition of the phages. After SDS-PAGE separation of phage virions digests 15 protein bands w"@en . . . "7" . "bacteriophage; phage therapy; MALDI-TOF; proteomics; genome"@en . "8"^^ . "Anal\u00FDza genomu a proteomu polyvalentn\u00EDho stafylokokov\u00E9ho bakteriof\u00E1ga 812" . "1210-6267" . . . . . "CZ - \u010Cesk\u00E1 republika" . "Preisler, Jan" . . . . "Zdr\u00E1hal, Zbyn\u011Bk" . . . "Pant\u016F\u010Dek, Roman" . "512227" . "R\u016F\u017Ei\u010Dkov\u00E1, Vladislava" . "Eyer, Lud\u011Bk" . "Genomic and proteomic characterization of polyvalent staphylococcal bacteriophage 812"@en . . "P(GA203/03/0515), Z(MSM0021622415)" . . "Anal\u00FDza genomu a proteomu polyvalentn\u00EDho stafylokokov\u00E9ho bakteriof\u00E1ga 812"@cs . "Informa\u010Dn\u00ED listy \u010Cesko-Slovensk\u00E9 genetick\u00E9 spole\u010Dnosti Gregora Mendela." . . . "Ka\u0161p\u00E1rek, Petr" . "Bakteriof\u00E1g 812, z\u00E1stupce \u010Deledi Myoviridae, je virulentn\u00ED polyvalentn\u00ED f\u00E1g s \u0161irok\u00FDm rozmez\u00EDm hostitel\u016F, zahrnuj\u00EDc\u00EDm kmeny a druhy rodu Staphylococcus. Tyto vlastnosti jej \u010Din\u00ED vhodn\u00FDm kandid\u00E1tem pro f\u00E1govou terapii stafylokokov\u00FDch infekc\u00ED. Tato pr\u00E1ce byla zam\u011B\u0159ena na srovn\u00E1n\u00ED genomu a proteomu standardn\u00EDho typu f\u00E1ga 812, jeho mutant v rozmez\u00ED hostitele a p\u0159\u00EDbuzn\u00FDch f\u00E1g\u016F U16, SK311, 131 a Twort s c\u00EDlem objastnit molekul\u00E1rn\u00ED z\u00E1klad jejich rozd\u00EDln\u00E9ho hostitelsk\u00E9ho rozmez\u00ED. Genomy uveden\u00FDch f\u00E1g\u016F tvo\u0159en\u00E9 line\u00E1rn\u00ED dvou\u0159et\u011Bzcovou DNA o velikosti 145 kb byly srovn\u00E1v\u00E1ny pomoc\u00ED restrik\u010Dn\u00ED anal\u00FDzy. \u00DAseky vykazuj\u00EDc\u00ED u jednotliv\u00FDch f\u00E1g\u016F rozd\u00EDly byly pak charakterizov\u00E1ny detailn\u011B. Pro \u00FA\u010Dely proteomick\u00FDch studi\u00ED byla pou\u017Eita 1-D SDS-PAGE. Nalezen\u00E9 proteiny byly identifikov\u00E1ny metodou peptidov\u00E9ho mapov\u00E1n\u00ED s vyu\u017Eit\u00EDm MALDI-TOF hmotnostn\u00ED spektrometrie. Srovn\u00E1n\u00EDm f\u00E1gov\u00FDch genom\u016F byly zji\u0161t\u011Bny vedle nukleotidov\u00FDch substituc\u00ED t\u00E9\u017E rozs\u00E1hlej\u0161\u00ED p\u0159estavby sekvenc\u00ED DNA. Inzerce a delece o velikosti ~1 a\u017E 3 kb byly nalezeny"@cs . "Kone\u010Dn\u00E1, Hana" . "14310" . "Genomic and proteomic characterization of polyvalent staphylococcal bacteriophage 812"@en . . "1" . . "28" . "Anal\u00FDza genomu a proteomu polyvalentn\u00EDho stafylokokov\u00E9ho bakteriof\u00E1ga 812" . . . . "RIV/00216224:14310/05:00012379" . "Anal\u00FDza genomu a proteomu polyvalentn\u00EDho stafylokokov\u00E9ho bakteriof\u00E1ga 812"@cs . "RIV/00216224:14310/05:00012379!RIV08-MSM-14310___" . . "1"^^ . "[3008179C4A7F]" . "Do\u0161ka\u0159, Ji\u0159\u00ED" . "Bakteriof\u00E1g 812, z\u00E1stupce \u010Deledi Myoviridae, je virulentn\u00ED polyvalentn\u00ED f\u00E1g s \u0161irok\u00FDm rozmez\u00EDm hostitel\u016F, zahrnuj\u00EDc\u00EDm kmeny a druhy rodu Staphylococcus. Tyto vlastnosti jej \u010Din\u00ED vhodn\u00FDm kandid\u00E1tem pro f\u00E1govou terapii stafylokokov\u00FDch infekc\u00ED. Tato pr\u00E1ce byla zam\u011B\u0159ena na srovn\u00E1n\u00ED genomu a proteomu standardn\u00EDho typu f\u00E1ga 812, jeho mutant v rozmez\u00ED hostitele a p\u0159\u00EDbuzn\u00FDch f\u00E1g\u016F U16, SK311, 131 a Twort s c\u00EDlem objastnit molekul\u00E1rn\u00ED z\u00E1klad jejich rozd\u00EDln\u00E9ho hostitelsk\u00E9ho rozmez\u00ED. Genomy uveden\u00FDch f\u00E1g\u016F tvo\u0159en\u00E9 line\u00E1rn\u00ED dvou\u0159et\u011Bzcovou DNA o velikosti 145 kb byly srovn\u00E1v\u00E1ny pomoc\u00ED restrik\u010Dn\u00ED anal\u00FDzy. \u00DAseky vykazuj\u00EDc\u00ED u jednotliv\u00FDch f\u00E1g\u016F rozd\u00EDly byly pak charakterizov\u00E1ny detailn\u011B. Pro \u00FA\u010Dely proteomick\u00FDch studi\u00ED byla pou\u017Eita 1-D SDS-PAGE. Nalezen\u00E9 proteiny byly identifikov\u00E1ny metodou peptidov\u00E9ho mapov\u00E1n\u00ED s vyu\u017Eit\u00EDm MALDI-TOF hmotnostn\u00ED spektrometrie. Srovn\u00E1n\u00EDm f\u00E1gov\u00FDch genom\u016F byly zji\u0161t\u011Bny vedle nukleotidov\u00FDch substituc\u00ED t\u00E9\u017E rozs\u00E1hlej\u0161\u00ED p\u0159estavby sekvenc\u00ED DNA. Inzerce a delece o velikosti ~1 a\u017E 3 kb byly nalezeny" . . .