"Malic enzymes of Trichomonas vaginalis: two enzyme families, two distinct origins"@en . . . "329" . . . "March" . "[7992A0C51277]" . "Tachezy, Jan" . "14310" . . "Va\u0148\u00E1\u010Dov\u00E1, \u0160t\u011Bp\u00E1nka" . "Hrd\u00FD, Ivan" . . . "RIV/00216224:14310/04:00036235" . . "The cytosolic malic enzyme of the amitochondriate protist Trichomonas vaginalis was purified to homogeneity and characterized. The corresponding gene was sequenced and compared with its hydrogenosomal homologue from the same organism. The enzymes were found to differ in coenzyme specificity, molecular mass and physiological role. The cytosolic malic enzyme is a dimer consisting of two 42-kDa subunits with strict specificity for nicotinamide adenine dinucleotide phosphate (NADP(+)), and has a presumed function of pyruvate and NADPH production. The hydrogenosomal malic enzyme is a tetramer of 60-kDa subunits that preferentially utilizes nicotinamide adenine dinucleotide (NAD(+)) to NADP(+). The hydrogenosomal enzyme supplies the hydrogenosome with pyruvate for further catabolic processes linked with substrate-level phosphorylation. Phylogenetic analysis of malic enzymes showed the existence of two distinct families of these enzymes in nature, which differ in subunit size." . "Trichomonas; Tritrichomonas; hydrogenosome; malic enzyme"@en . "V, Z(MSM 113100003)" . "572147" . . . . . "0378-1119" . "Malic enzymes of Trichomonas vaginalis: two enzyme families, two distinct origins" . . "Gene" . . "12"^^ . "Malic enzymes of Trichomonas vaginalis: two enzyme families, two distinct origins"@en . "RIV/00216224:14310/04:00036235!RIV10-MSM-14310___" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "1"^^ . . . "Malic enzymes of Trichomonas vaginalis: two enzyme families, two distinct origins" . "Dolezal, Pavel" . "4"^^ . . "The cytosolic malic enzyme of the amitochondriate protist Trichomonas vaginalis was purified to homogeneity and characterized. The corresponding gene was sequenced and compared with its hydrogenosomal homologue from the same organism. The enzymes were found to differ in coenzyme specificity, molecular mass and physiological role. The cytosolic malic enzyme is a dimer consisting of two 42-kDa subunits with strict specificity for nicotinamide adenine dinucleotide phosphate (NADP(+)), and has a presumed function of pyruvate and NADPH production. The hydrogenosomal malic enzyme is a tetramer of 60-kDa subunits that preferentially utilizes nicotinamide adenine dinucleotide (NAD(+)) to NADP(+). The hydrogenosomal enzyme supplies the hydrogenosome with pyruvate for further catabolic processes linked with substrate-level phosphorylation. Phylogenetic analysis of malic enzymes showed the existence of two distinct families of these enzymes in nature, which differ in subunit size."@en .