. . "[3579390BFBC5]" . "Jak vyhodnocovat odpov\u011B\u010F cytoskeletu na \u00FA\u010Dinky ultrazvuku?"@cs . "126" . . . . . . "Be\u010Dv\u00E1\u0159ov\u00E1, Pavl\u00EDna" . . "How to evaluate the cytoskeletal response to the action of ultrasound?"@en . "Nov\u00FD, Jind\u0159ich" . "566749" . "5"^^ . "P(GA301/01/1102)" . "Cytoskeleton changes are usually qualitatively described in vague terms like rarefaction, aggregation etc. An objective method for quantifying cytoskeletal changes was not yet published. We have used HeLa cells cultured in a standard way. Cells were exposed to 1 MHz ultrasound (1 and 2 W.cm-2) for 10 min. The microtubules were detected by a fluorescence microscope after by immunofluorescence method. Method of cell structure analysis is based on frequency domain filtering using the discrete Fourier transform. We designed filters enabling extraction of significant information about structural changes of microtubules from micrographs. For comparison purposes we applied further analysis which assesses the degree of damage of microtubules and assigns it a numeric descriptor computed as the ratio of area occupied by microtubules to the total area of the cell. Our mathematical approach was sufficiently sensitive for assessment of microtubular network changes." . "Lije\u010Dni\u010Dki Vjesnik" . "Ultrasound; cytoskeleton; immunofluorescence; fast Fourier transform; microtubules; image analysis"@en . . . . "14310" . "Janisch, Roman" . "1"^^ . "RIV/00216224:14310/04:00011664" . . . "Mornstein, Vojt\u011Bch" . "Supp. 2" . . "RIV/00216224:14310/04:00011664!RIV08-GA0-14310___" . "How to evaluate the cytoskeletal response to the action of ultrasound?" . "\u0160korp\u00EDkov\u00E1, Ji\u0159ina" . "Zm\u011Bny cytoskeletu jsou obvykle kvalitativn\u011B popisov\u00E1ny pomoc\u00ED v\u00E1gn\u00EDch term\u00EDn\u016F jako z\u0159ed\u011Bn\u00ED, agregace apod. Objektivn\u00ED metoda pro kvantifikaci cytoskelet\u00E1ln\u00EDch zm\u011Bn nebyla dosud publikov\u00E1na. Pou\u017Eili jsme bu\u0148ky HeLa p\u011Bstovan\u00E9 standardn\u00EDm zp\u016Fsobem. Bu\u0148ky byly vystaveny \u00FA\u010Dink\u016Fm ultrazvuku o frekvenci 1 MHz (1 a 2 W.cm-2) na dobu 10 min. Mikrotubuly byly detekov\u00E1ny fluorescen\u010Dn\u00EDm mikroskopem imunofluorescen\u010Dn\u00ED metodou. Metody strukturn\u00ED anal\u00FDzy bun\u011Bk je zalo\u017Eena na filtrov\u00E1n\u00ED frekven\u010Dn\u00EDch dom\u00E9n pomoc\u00ED diskr\u00E9tn\u00ED Fourierovy transformace. Byly p\u0159ipraveny filtry umo\u017E\u0148uj\u00ED extrakci v\u00FDznamn\u00E9 informace o strukturn\u00EDch zm\u011Bn\u00E1ch mikrotubul\u016F z mikrofotografi\u00ED. Pro srovn\u00E1vac\u00ED \u00FA\u010Dely jsme pou\u017Eili dal\u0161\u00ED anal\u00FDzu hodnot\u00EDc\u00ED stupe\u0148 po\u0161kozen\u00ED mikrotubul\u016F a p\u0159i\u0159azuj\u00EDc\u00ED mu numerick\u00FD deskriptor po\u010D\u00EDtan\u00FD jako pom\u011Br plochy zauj\u00EDman\u00E9 mikrotubuly a celkov\u00E9 plochy bu\u0148ky. N\u00E1\u0161 matematick\u00FD p\u0159\u00EDstup byl dostate\u010Dn\u011B citliv\u00FD pro posouzen\u00ED zm\u011Bn s\u00EDt\u011B mikrotubul\u016F."@cs . "168-168" . . "Jak vyhodnocovat odpov\u011B\u010F cytoskeletu na \u00FA\u010Dinky ultrazvuku?"@cs . . "How to evaluate the cytoskeletal response to the action of ultrasound?"@en . "How to evaluate the cytoskeletal response to the action of ultrasound?" . "1330-4917" . . "HR - Chorvatsk\u00E1 republika" . . . "Cytoskeleton changes are usually qualitatively described in vague terms like rarefaction, aggregation etc. An objective method for quantifying cytoskeletal changes was not yet published. We have used HeLa cells cultured in a standard way. Cells were exposed to 1 MHz ultrasound (1 and 2 W.cm-2) for 10 min. The microtubules were detected by a fluorescence microscope after by immunofluorescence method. Method of cell structure analysis is based on frequency domain filtering using the discrete Fourier transform. We designed filters enabling extraction of significant information about structural changes of microtubules from micrographs. For comparison purposes we applied further analysis which assesses the degree of damage of microtubules and assigns it a numeric descriptor computed as the ratio of area occupied by microtubules to the total area of the cell. Our mathematical approach was sufficiently sensitive for assessment of microtubular network changes."@en . . . "4"^^ .