"\u00DAstav \u017Eivo\u010Di\u0161n\u00E9 fyziologie a genetiky AV \u010CR. St\u0159edisko v\u011Bdeckotechnick\u00FDch informac\u00ED" . . . "RIV/00216224:14310/04:00011469" . "14310" . . . "\u0160marda, Jan" . . . "Sample preparation for proteom analysis of leukemic cells BM2 using two-dimensional electrophoresis"@en . "P\u0159\u00EDprava vzorku pro anal\u00FDzu proteomu leukemick\u00FDch bun\u011Bk BM2 dvourozm\u011Brnou elektrofor\u00E9zou"@cs . . . "P\u0159\u00EDprava vzorku pro anal\u00FDzu proteomu leukemick\u00FDch bun\u011Bk BM2 dvourozm\u011Brnou elektrofor\u00E9zou" . . . . "P\u0159\u00EDprava vzorku pro anal\u00FDzu proteomu leukemick\u00FDch bun\u011Bk BM2 dvourozm\u011Brnou elektrofor\u00E9zou"@cs . "[2DB3AE63D870]" . "Quality of two-dimensional (2D) electrophoresis is dependent on the way of sample preparation. The aim of this study was to set up optimal conditions for preparation of the sample of v-myb-transformed chicken BM2 monoblasts to get a high number of resolved proteins in 2D gel. Starting composition of both lysis (9 M urea, 2 % NP-40, 3 % CHAPS, 70 mM DTT and 2 % ampholyte pH 8-10.5) and rehydratation buffers (8 M urea, 4 % CHAPS, 100 mM DTT, 0.001 % bromphenol blue and 0.2 % ampholyte pH 3-10) have been modified by addition of thiourea causing clear increase of the number of protein spots detectable in 2D gel. In addition, sonication of the sample further increased the number and density of resolved proteins. The presence of thiourea in the lysis/rehydratation buffers and sonication increased the number of protein spots resolved in 2D gel about 8-fold. These experimental conditions are going to be used in analysis of proteome during terminal differentiation of BM2 monoblasts."@en . "581939" . . . "M\u011Bln\u00EDk" . "M\u011Bln\u00EDk" . "V\u00FDsledek dvourozm\u011Brn\u00E9 elektrofor\u00E9zy (2D) protein\u016F v\u00FDnamn\u011B z\u00E1vis\u00ED na zp\u016Fsobu p\u0159\u00EDpravy vzorku. C\u00EDlem t\u00E9to pr\u00E1ce bylo stanovit optim\u00E1ln\u00ED podm\u00EDnky pro p\u0159\u00EDpravu vzorku ku\u0159ec\u00EDch monoblast\u016F transormovan\u00FDch onkogenem v-myb. Modifikac\u00ED lyza\u010Dn\u00EDho (9 M urea, 2 % NP-40, 3 % CHAPS, 70 mM DTT, 2 % ampholyte pH 8-10.5) a rehydrata\u010Dn\u00EDho pufru (8 M urea, 4 % CHAPS, 100 mM DTT, 0.001 % bromphenol blue, 0.2 % ampholyte pH 3-10)p\u0159id\u00E1n\u00EDm thiomo\u010Doviny jsme z\u0159eteln\u011B zv\u00FD\u0161ili po\u010Det protein\u016F rozli\u0161iteln\u00FDch v 2D gelu. Sonikac\u00ED vzorku jsme d\u00E1le zv\u00FD\u0161ili po\u010Det a densitu protein\u016F na elektroforetogramu. P\u0159\u00EDtomnost thiomo\u010Doviny v lyza\u010Dn\u00EDm/rehydrata\u010Dn\u00EDm pufru a sonikace zv\u00FD\u0161ila po\u010Det rozli\u0161iteln\u00FDch proteinov\u00FDch skvrn p\u0159ibli\u017En\u011B osmkr\u00E1t. Tyto experiment\u00E1ln\u00ED podm\u00EDnky pou\u017Eijeme p\u0159i anal\u00FDze proteomu b\u011Bhem diferenciace monoblast\u016F BM2." . . . "2"^^ . "24" . . "Sample preparation for proteom analysis of leukemic cells BM2 using two-dimensional electrophoresis"@en . "RIV/00216224:14310/04:00011469!RIV08-GA0-14310___" . "1"^^ . "1. \u010Desk\u00E1 proteomick\u00E1 konference" . "Two-dimensional electrophoresis; proteome analysis; sample preparation"@en . "P\u0159\u00EDprava vzorku pro anal\u00FDzu proteomu leukemick\u00FDch bun\u011Bk BM2 dvourozm\u011Brnou elektrofor\u00E9zou" . "V\u00FDsledek dvourozm\u011Brn\u00E9 elektrofor\u00E9zy (2D) protein\u016F v\u00FDnamn\u011B z\u00E1vis\u00ED na zp\u016Fsobu p\u0159\u00EDpravy vzorku. C\u00EDlem t\u00E9to pr\u00E1ce bylo stanovit optim\u00E1ln\u00ED podm\u00EDnky pro p\u0159\u00EDpravu vzorku ku\u0159ec\u00EDch monoblast\u016F transormovan\u00FDch onkogenem v-myb. Modifikac\u00ED lyza\u010Dn\u00EDho (9 M urea, 2 % NP-40, 3 % CHAPS, 70 mM DTT, 2 % ampholyte pH 8-10.5) a rehydrata\u010Dn\u00EDho pufru (8 M urea, 4 % CHAPS, 100 mM DTT, 0.001 % bromphenol blue, 0.2 % ampholyte pH 3-10)p\u0159id\u00E1n\u00EDm thiomo\u010Doviny jsme z\u0159eteln\u011B zv\u00FD\u0161ili po\u010Det protein\u016F rozli\u0161iteln\u00FDch v 2D gelu. Sonikac\u00ED vzorku jsme d\u00E1le zv\u00FD\u0161ili po\u010Det a densitu protein\u016F na elektroforetogramu. P\u0159\u00EDtomnost thiomo\u010Doviny v lyza\u010Dn\u00EDm/rehydrata\u010Dn\u00EDm pufru a sonikace zv\u00FD\u0161ila po\u010Det rozli\u0161iteln\u00FDch proteinov\u00FDch skvrn p\u0159ibli\u017En\u011B osmkr\u00E1t. Tyto experiment\u00E1ln\u00ED podm\u00EDnky pou\u017Eijeme p\u0159i anal\u00FDze proteomu b\u011Bhem diferenciace monoblast\u016F BM2."@cs . "2004-06-15+02:00"^^ . "V\u00E1\u0148a, Petr" . "2"^^ . . "P(GA301/03/1055)" .