"14310" . . . "RIV/00216224:14310/04:00011440" . "12"^^ . "2004-02-08+01:00"^^ . . . "Nov\u00E1kov\u00E1, Jarom\u00EDra" . . "Pant\u016F\u010Dek, Roman" . "Foret, Franti\u0161ek" . "Do\u0161ka\u0159, Ji\u0159\u00ED" . "Kapil\u00E1rn\u00ED elektrofor\u00E9za protein\u016F bakteriof\u00E1ga 812 kombinovan\u00E1 s MALDI-hmotnostn\u00ED spektrometri\u00ED"@cs . . . "Salzburg, Austria" . . "Salzburg, Austria" . . . . "Eyer, Lud\u011Bk" . "Capillary Electrophoresis of Bacteriophage 812 Proteins Combined with MALDI Mass Spectrometry"@en . . . "HPCE" . . "P(GA203/03/0515)" . "Sp\u011B\u0161n\u00FD, Michal" . "The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. In this work, SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis.Additionally, we have proposed a new approach to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. Protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS. A method of protein microdigestion on MALDI target is being studied and will be used for protein identification."@en . . . . "Capillary Electrophoresis of Bacteriophage 812 Proteins Combined with MALDI Mass Spectrometry" . . "Capillary Electrophoresis of Bacteriophage 812 Proteins Combined with MALDI Mass Spectrometry"@en . "Kapil\u00E1rn\u00ED elektrofor\u00E9za protein\u016F bakteriof\u00E1ga 812 kombinovan\u00E1 s MALDI-hmotnostn\u00ED spektrometri\u00ED"@cs . "The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. In this work, SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis.Additionally, we have proposed a new approach to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. Protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS. A method of protein microdigestion on MALDI target is being studied and will be used for protein identification." . "556818" . "2"^^ . "Kone\u010Dn\u00E1, Hana" . "Kr\u00E1sensk\u00FD, Pavel" . "Capillary Electrophoresis of Bacteriophage 812 Proteins Combined with MALDI Mass Spectrometry" . . . . "Vr\u00E1bel, Patrik" . . "Capillary Electrophoresis; Proteins; Bacteriophage"@en . "Zdr\u00E1hal, Zbyn\u011Bk" . . "Klep\u00E1rn\u00EDk, Karel" . . "RIV/00216224:14310/04:00011440!RIV09-GA0-14310___" . . "[3349DE64B5CF]" . "The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. In this work, SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis.Additionally, we have proposed a new approach to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. Protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS. A method of protein microdigestion on MALDI target is being studied and will be used for protein identification. Anotace anglicky: The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. In this work, SDS gel electrophoresis was used for the protei"@cs . "Preisler, Jan" . . "Final Program and Book of Abstracts of 17th International Symposium on Microscale Separations and Capillary Electrophoresis" . "11"^^ .