"RIV/00216224:14310/04:00010825!RIV08-MSM-14310___" . "In our case study, we measured the functional diversity of the microbial communities of twelve soils from the small natural area to assess if this assay is suitable for routine monitoring of soil biological quality. We found the BIOLOG assay meets especially practical benefits in routine monitoring of soils being simple and quick assay. However, we confirmed the ambiguity about the most appropriate analysis of the BIOLOG multivariate data and about the best parameter, which can be derived from the assay. The different analyses of the data were examined and various parameters derived from the BIOLOG assay were comparatively used to contribute to the discussion of how the data should be evaluated. We showed that not-normalized raw absorbances or trapezoid areas should be used for calculation of diversity index if the inoculum was standardized. There was no single answer to what parameter provided more correct results in the multivariate statistical analysis. Evaluating at least one not-normalized (e.g." . "50" . "Evaluation of functional diversity of soil microbial communities - a case study"@en . "Hofman, Jakub" . . . . "BIOLOG; soil microorganisms; soil monitoring; functional diversity"@en . "In our case study, we measured the functional diversity of the microbial communities of twelve soils from the small natural area to assess if this assay is suitable for routine monitoring of soil biological quality. We found the BIOLOG assay meets especially practical benefits in routine monitoring of soils being simple and quick assay. However, we confirmed the ambiguity about the most appropriate analysis of the BIOLOG multivariate data and about the best parameter, which can be derived from the assay. The different analyses of the data were examined and various parameters derived from the BIOLOG assay were comparatively used to contribute to the discussion of how the data should be evaluated. We showed that not-normalized raw absorbances or trapezoid areas should be used for calculation of diversity index if the inoculum was standardized. There was no single answer to what parameter provided more correct results in the multivariate statistical analysis. Evaluating at least one not-normalized (e.g."@en . . . "Evaluation of functional diversity of soil microbial communities - a case study"@en . "Evaluation of functional diversity of soil microbial communities - a case study" . . . . . "RIV/00216224:14310/04:00010825" . . . "Holoubek, Ivan" . "8"^^ . "3"^^ . . "4" . "Evaluation of functional diversity of soil microbial communities - a case study" . "Plant Soil and Environment" . . "3"^^ . . "[BDD1A76663AA]" . . . "Hodnocen\u00ED funk\u010Dn\u00ED diverzity p\u016Fdn\u00EDch mikrobi\u00E1ln\u00EDch spole\u010Denstev - p\u0159\u00EDpadov\u00E1 studie"@cs . . "0370-663X" . "V t\u00E9to p\u0159\u00EDpadov\u00E9 studii jsme m\u011B\u0159ili funk\u010Dn\u00ED diverzitu p\u016Fdn\u00EDch mikrobi\u00E1ln\u00EDch spole\u010Denstev dvan\u00E1cti p\u016Fd z mal\u00E9 p\u0159\u00EDrodn\u00ED oblasti s c\u00EDlem posoudit vhodnost pou\u017Eit\u00ED tohoto p\u0159\u00EDstupu pro rutinn\u00ED monitoring p\u016Fdn\u00ED biologick\u00E9 kvality. Shledali jsme, \u017Ee metoda BIOLOG spl\u0148uje zejm\u00E9na praktick\u00E9 v\u00FDhody pro rutinn\u00ED monitoring p\u016Fd, proto\u017Ee je rychl\u00E1 a jednoduch\u00E1. Na druhou stranu jsme potvrdili nejednozna\u010Dnost kolem nejvhodn\u011Bj\u0161\u00ED anal\u00FDzy v\u00EDcerozm\u011Brn\u00FDch dat z pou\u017Eit\u00E9 metody. Tak\u00E9 v\u00FDb\u011Br nejlep\u0161\u00EDho parametru metody je problematick\u00FD. Abychom p\u0159isp\u011Bli k diskusi, jak maj\u00ED b\u00FDt data z metody BIOLOG hodnocena, pou\u017Eili jsme pro srovn\u00E1n\u00ED r\u016Fzn\u00E9 parametry a zp\u016Fsoby jejich vyhodnocen\u00ED. Uk\u00E1zalo se, \u017Ee pokud bylo inokulum standardizov\u00E1no, m\u011Bly by b\u00FDt pro v\u00FDpo\u010Det indexu diverzity pou\u017Eity nenormalizovan\u00E9 absorbance \u010Di trapezoid areas. Co se t\u00FDk\u00E1 v\u00EDcerozm\u011Brn\u00FDch statistick\u00FDch anal\u00FDz, nelze jednozna\u010Dn\u011B ur\u010Dit, kter\u00FD parametr je korektn\u011Bj\u0161\u00ED. Bylo doporu\u010Deno hodnocen\u00ED alespo\u0148 jednoho nenormalizovan\u00E9ho (nap\u0159. trapezoid area) a jednoho normali"@cs . . "P(GA525/03/0367), P(SI/340/1/00), Z(MSM 141100003)" . "14310" . . "563357" . . "141-148" . "CZ - \u010Cesk\u00E1 republika" . "Hodnocen\u00ED funk\u010Dn\u00ED diverzity p\u016Fdn\u00EDch mikrobi\u00E1ln\u00EDch spole\u010Denstev - p\u0159\u00EDpadov\u00E1 studie"@cs . . "\u0160vih\u00E1lek, Ji\u0159\u00ED" .