"2004-07-11+02:00"^^ . "Max Planck Institute of Molecular Plant Physiology" . . "Identification and characterisation of three genes determining embryogenesis by means of T-DNA mutagenesis in Arabidopsis thaliana."@en . "3"^^ . "Identification and characterisation of three genes determining embryogenesis by means of T-DNA mutagenesis in Arabidopsis thaliana."@en . "Identification and characterisation of three genes determining embryogenesis by means of T-DNA mutagenesis in Arabidopsis thaliana." . . . "T01-037" . . "2"^^ . "One of the possible approaches to a plant gene function study in model plant Arabidopsis thaliana leads across T-DNA mutagenesis. Collection of 2500 T-DNA lines have been obtained by A. thaliana transformation with Agrobacterium tumefaciens containing plasmid pPCVRN4 with HPT selectable gene (Koncz et al. 1994). Screening of individual lines revealed three different mutations with defects in immature seed development (marked 1265, 1293, 1321) which have been characterised and analysed in detail. One T-DNA insert was determined in each line and the mutations were confirmed to be monogenic and recessive-lethal by genetic analysis. Morphological studies of embryo development were performed by clearing in chloral hydrate. Mutant embryos were blocked in certain steps in the process necessary for embryo viability and development. In 1265 mutation defect in globular stage was observed. It was associated with abnormal pattern of cell division connected with shoot apical meristem formation during embryogenesi" . "Z(MSM 143100008)" . "Abstract Book of the 15th International Conference on Arabidopsis Research, July 11-14, Berlin, Germany." . "Koc\u00E1bek, Tom\u00E1\u0161" . . . "RIV/00216224:14310/04:00010788" . "Identifikace a characterizace t\u0159\u00ED gen\u016F determinuj\u00EDc\u00EDch embryogenezi prost\u0159ednictv\u00EDm T-DNA mutac\u00ED u Arabidopsis thaliana."@cs . . "One of the possible approaches to a plant gene function study in model plant Arabidopsis thaliana leads across T-DNA mutagenesis. Collection of 2500 T-DNA lines have been obtained by A. thaliana transformation with Agrobacterium tumefaciens containing plasmid pPCVRN4 with HPT selectable gene (Koncz et al. 1994). Screening of individual lines revealed three different mutations with defects in immature seed development (marked 1265, 1293, 1321) which have been characterised and analysed in detail. One T-DNA insert was determined in each line and the mutations were confirmed to be monogenic and recessive-lethal by genetic analysis. Morphological studies of embryo development were performed by clearing in chloral hydrate. Mutant embryos were blocked in certain steps in the process necessary for embryo viability and development. In 1265 mutation defect in globular stage was observed. It was associated with abnormal pattern of cell division connected with shoot apical meristem formation during embryogenesi"@en . . . "Arabidopsis thaliana, T-DNA"@en . . "Berlin, Germany" . "1"^^ . "Berlin, Germany" . "Dudov\u00E1, Mark\u00E9ta" . "Identification and characterisation of three genes determining embryogenesis by means of T-DNA mutagenesis in Arabidopsis thaliana." . . "Jedn\u00EDm z mo\u017En\u00FDch p\u0159\u00EDstup\u016F studia funkc\u00ED rostlinn\u00FDch gen\u016F u Arabidopsis thaliana je T-DNA mutageneze. Kolekce 2500 T-DNA lini\u00ED po transformaci Agrobacterium tumefaciens byla vyu\u017Eita p\u0159i vyhled\u00E1v\u00E1n\u00ED mutac\u00ED s embryon\u00E1ln\u00EDmi defekty. T\u0159i tyto mutace (1265, 1293, 1321) podrobn\u011B analyzov\u00E1ny a charakterizov\u00E1ny. Genetick\u00E1 anal\u00FDza potvrdila monogenn\u00ED a recesivn\u00ED charakter t\u011Bchto mutac\u00ED."@cs . "Identifikace a characterizace t\u0159\u00ED gen\u016F determinuj\u00EDc\u00EDch embryogenezi prost\u0159ednictv\u00EDm T-DNA mutac\u00ED u Arabidopsis thaliana."@cs . . "567128" . . . "[3870D9796B66]" . . "\u0158epkov\u00E1, Jana" . . "14310" . "RIV/00216224:14310/04:00010788!RIV/2005/MSM/143105/N" .