"Petr\u00E1\u0161, Petr" . . "5"^^ . . "1"^^ . "Bakteri\u00E1ln\u00ED druh Staphylococcus aureus produkuje exfoliativn\u00ED toxiny, kter\u00E9 zp\u016Fsobuj\u00ED u lid\u00ED a zejm\u00E9na u kojenc\u016F ko\u017En\u00ED puch\u00FD\u0159nat\u00E9 onemocn\u011Bn\u00ED, jeho\u017E nejt\u011B\u017E\u0161\u00ED formou je syndrom opa\u0159en\u00E9 k\u016F\u017Ee. Gen eta k\u00F3duj\u00EDc\u00ED toxin ETA se nach\u00E1z\u00ED v DNA prof\u00E1ga, co\u017E je genom m\u00EDrn\u00E9ho f\u00E1ga za\u010Dlen\u011Bn\u00FD do chromozomu hostitelsk\u00E9 bakterie. M\u00EDrn\u00E9 stafylof\u00E1gy lyzogenn\u00ED konverz\u00ED m\u011Bn\u00ED genetick\u00E9 a fenotypov\u00E9 vlastnosti hostitelsk\u00E9ho kmene. V t\u00E9to pr\u00E1ci byly izolov\u00E1ny bakteriof\u00E1gy z osmi ETA-pozitivn\u00EDch kmen\u016F S. aureus. Metodou multiplex PCR bylo p\u011Bt f\u00E1g\u016F za\u0159azeno do serologick\u00E9 skupiny A a t\u0159i f\u00E1gy do serologick\u00E9 skupiny B. PCR detekc\u00ED byl u nich prok\u00E1z\u00E1n strukturn\u00ED gen eta. V\u0161echny f\u00E1gy byly charakterizov\u00E1ny restrik\u010Dn\u00ED anal\u00FDzou DNA pomoc\u00ED restrikt\u00E1zy HindIII takto: ve skupin\u011B A-f\u00E1g\u016F byly rozli\u0161eny t\u0159i subtypy A-1, A-2 a A-3, zat\u00EDmco u B-f\u00E1g\u016F byly rozli\u0161eny dva subtypy, B-1a B-2. T\u0159i f\u00E1gy byly pou\u017Eity k p\u0159enosu genu eta do recipietn\u00EDho kmene. Jeden A-f\u00E1g a dva B-f\u00E1gy lyzogenizovaly ETA-negativn\u00ED kmen S. aureus. Bylo zji\u0161t\u011Bno, \u017Ee gen"@cs . "Charleston, South Carolina, USA" . . "Two exfoliative toxins, ETA and ETB, produced by certain strains of S. aureus are the major causative agents of blistering skin disorders with the most severe form the Staphylococcal Scalded Skin Syndrome, primarily affecting infants and young children. The eta gene encoding ETA is located on the chromosome, whereas the etb gene encoding ETB is located on a large plasmid. Temperate bacteriophages carrying the eta gene and converting exfoliative toxin A production in S. aureus, have been recently, found. Eight bacteriophages isolated from 8 human ETA-positive strains of S. aureus by UV induction were classified into serogroups, screened for carriage of the eta gene by PCR, and characterized by HindIII restriction analysis. Three of them were used for the ETA conversion experiments. Location of prophages in lysogens was revealed by PFGE and ETA production was detected by RPLA. Five of the phages were classified into serogroup A (A-phages) and three into serogroup B (B-phages). Restriction enzyme HindIII"@en . "Charleston, South Carolina, USA" . . . . . "R\u016F\u017Ei\u010Dkov\u00E1, Vladislava" . . . "Two exfoliative toxins, ETA and ETB, produced by certain strains of S. aureus are the major causative agents of blistering skin disorders with the most severe form the Staphylococcal Scalded Skin Syndrome, primarily affecting infants and young children. The eta gene encoding ETA is located on the chromosome, whereas the etb gene encoding ETB is located on a large plasmid. Temperate bacteriophages carrying the eta gene and converting exfoliative toxin A production in S. aureus, have been recently, found. Eight bacteriophages isolated from 8 human ETA-positive strains of S. aureus by UV induction were classified into serogroups, screened for carriage of the eta gene by PCR, and characterized by HindIII restriction analysis. Three of them were used for the ETA conversion experiments. Location of prophages in lysogens was revealed by PFGE and ETA production was detected by RPLA. Five of the phages were classified into serogroup A (A-phages) and three into serogroup B (B-phages). Restriction enzyme HindIII" . "P(GA301/02/1505), Z(MSM 143100008)" . . "Characterization of resident bacteriophages and their role in conversion of exfoliative toxin A production in Staphylococcus aureus" . . "11th International Symposium on Staphylococci & Staphylococcal Infections. Plenary Summaries & Poster Abstracts" . . "staphylococcus; exfoliative toxin A; bacteriophage; lysogenic conversion"@en . . "557418" . "RIV/00216224:14310/04:00010415" . "[BDB09E3F94E6]" . . . "Medical University of South Carolina" . . "2004-10-24+02:00"^^ . . . "Charakterizace rezidentn\u00EDch bakteriof\u00E1g\u016F a jejich role v konverzi exfoliatin A produkuj\u00EDc\u00EDch kmen\u016F Staphylococcus aureus"@cs . "Do\u0161ka\u0159, Ji\u0159\u00ED" . "14310" . "Charakterizace rezidentn\u00EDch bakteriof\u00E1g\u016F a jejich role v konverzi exfoliatin A produkuj\u00EDc\u00EDch kmen\u016F Staphylococcus aureus"@cs . "Characterization of resident bacteriophages and their role in conversion of exfoliative toxin A production in Staphylococcus aureus"@en . "4"^^ . . "Pant\u016F\u010Dek, Roman" . "Characterization of resident bacteriophages and their role in conversion of exfoliative toxin A production in Staphylococcus aureus" . . . "Characterization of resident bacteriophages and their role in conversion of exfoliative toxin A production in Staphylococcus aureus"@en . "Pekarov\u00E1, M\u00E1ria" . "RIV/00216224:14310/04:00010415!RIV09-GA0-14310___" . .