"14310" . . "Pant\u016F\u010Dek, Roman" . . "Do\u0161ka\u0159, Ji\u0159\u00ED" . "staphylococcus; bacteriophage"@en . "Virulent and polyvalent staphylophage 812, a member of Myoviridae family, exhibits strong lytic activity on a broad host range of Staphylococcus species, thus being a suitable candidate for treatment of staphylococcal infections. Genome of this phage is represented by linear 145 kb dsDNA. Our study was focused on genome and proteome comparison of 812 wild-type phage, its host-range mutants and related phages U16, 131 and SK311 with the aim to explain molecular basis of differences in their host-range. The genomes of phages under study were compared by DNA restriction analysis, and the genomic regions showing differences in individual phages were characterized in details. The proteome analysis included separation of phage protein lysates by 1D SDS-PAGE with subsequent identification of proteins by peptide mapping using MALDI-TOF mass spectrometry. Comparison of the phage genomes revealed several rearrangements of DNA sequences as well as single nucleotide substitutions in different parts of the genomes" . "R\u016F\u017Ei\u010Dkov\u00E1, Vladislava" . . . . . . . . "1"^^ . . . "RIV/00216224:14310/04:00010413" . "Kone\u010Dn\u00E1, Hana" . "Charakterizace genomu a proteomu polyvalentn\u00EDho f\u00E1ga 812"@cs . "Preisler, Jan" . "Genomic and proteomic characterization of the polyvalent staphylophage 812" . "Ka\u0161p\u00E1rek, Petr" . "RIV/00216224:14310/04:00010413!RIV09-GA0-14310___" . . . "Genomic and proteomic characterization of the polyvalent staphylophage 812" . . "P(GA203/03/0515), Z(MSM 143100008)" . . . "Genomic and proteomic characterization of the polyvalent staphylophage 812"@en . "Bakteriof\u00E1g 812, z\u00E1stupce \u010Deledi Myoviridae, je virulentn\u00ED polyvalentn\u00ED f\u00E1g s \uFFFDirok\u00FDm rozmez\u00EDm hostitel\u016F, zahrnuj\u00EDc\u00EDm kmeny a druhy rodu Staphylococcus. Tyto vlastnosti jej \u010Din\u00ED vhodn\u00FDm kandid\u00E1tem pro f\u00E1govou terapii stafylokokov\u00FDch infekc\u00ED. Tato pr\u00E1ce byla zam\u011B\u0159ena na srovn\u00E1n\u00ED genomu a proteomu standardn\u00EDho typu f\u00E1ga 812, jeho mutant v rozmez\u00ED hostitele a p\u0159\u00EDbuzn\u00FDch f\u00E1g\u016F U16, SK311, 131 a Twort s c\u00EDlem objastnit molekul\u00E1rn\u00ED z\u00E1klad jejich rozd\u00EDln\u00E9ho hostitelsk\u00E9ho rozmez\u00ED. Genomy uveden\u00FDch f\u00E1g\u016F tvo\u0159en\u00E9 line\u00E1rn\u00ED dvou\u0159et\u011Bzcovou DNA o velikosti 145 kb byly srovn\u00E1v\u00E1ny pomoc\u00ED restrik\u010Dn\u00ED anal\u00FDzy. \u00DAseky vykazuj\u00EDc\u00ED u jednotliv\u00FDch f\u00E1g\u016F rozd\u00EDly byly pak charakterizov\u00E1ny detailn\u011B. Pro \u00FA\u010Dely proteomick\u00FDch studi\u00ED byla pou\uFFFDita 1-D SDS-PAGE. Nalezen\u00E9 proteiny byly identifikov\u00E1ny metodou peptidov\u00E9ho mapov\u00E1n\u00ED s vyu\uFFFDit\u00EDm MALDI-TOF hmotnostn\u00ED spektrometrie. Srovn\u00E1n\u00EDm f\u00E1gov\u00FDch genom\u016F byly zji\uFFFDt\u011Bny vedle nukleotidov\u00FDch substituc\u00ED t\u00E9\uFFFD rozs\u00E1hlej\uFFFD\u00ED p\u0159estavby sekvenc\u00ED DNA. Inzerce a delece o velikosti ~1 a\uFFFD 3 kb byly nalezeny"@cs . . "Charleston, South Carolina, USA" . "[62E5C4C4F587]" . "Charleston, South Carolina, USA" . "Genomic and proteomic characterization of the polyvalent staphylophage 812"@en . . "Virulent and polyvalent staphylophage 812, a member of Myoviridae family, exhibits strong lytic activity on a broad host range of Staphylococcus species, thus being a suitable candidate for treatment of staphylococcal infections. Genome of this phage is represented by linear 145 kb dsDNA. Our study was focused on genome and proteome comparison of 812 wild-type phage, its host-range mutants and related phages U16, 131 and SK311 with the aim to explain molecular basis of differences in their host-range. The genomes of phages under study were compared by DNA restriction analysis, and the genomic regions showing differences in individual phages were characterized in details. The proteome analysis included separation of phage protein lysates by 1D SDS-PAGE with subsequent identification of proteins by peptide mapping using MALDI-TOF mass spectrometry. Comparison of the phage genomes revealed several rearrangements of DNA sequences as well as single nucleotide substitutions in different parts of the genomes"@en . "Zdr\u00E1hal, Zbyn\u011Bk" . "8"^^ . . "Charakterizace genomu a proteomu polyvalentn\u00EDho f\u00E1ga 812"@cs . "565400" . "11th International Symposium on Staphylococci & Staphylococcal Infections. Plenary Summaries & Poster Abstracts" . . . "Medical University of South Carolina" . "2004-10-24+02:00"^^ . "8"^^ . . "Eyer, Lud\u011Bk" . . .